Nikolaev A A, Lutskiĭ D L, Bochanovskiĭ V A, Lozhkina L V
Urol Nefrol (Mosk). 1997 Sep-Oct(5):35-9.
Enzymatic ejaculates activity was studied in 15 fertile and 65 subfertile samples of human sperm. The activity of alkaline phosphatase (EC 3.1.3.1), alpha-amylase (EC 3.2.1.1), gamma-glutamyl transferase (EC 2.3.2.2), creatine kinase (EC 2.7.3.2), total lactate dehydrogenase (EC 1.1.1.27) and lactate dehydrogenase-1 was compared to spermogram findings according to 14 morphological and physicochemical criteria of fertility. The enzyme activity depends on functioning of the testes, seminal vesiculae, prostate, bulbourethral glands and is involved in formation of fertility. The findings allowed elucidation of the role of synthesis and some function of spermoplasma enzymes. Practical applications of the above data are discussed in the treatment of male infertility and to raise fertilizing power of the sperm in artificial insemination.
对15份生育能力正常和65份生育能力低下的人类精液样本进行了酶活性研究。根据14项生育能力的形态学和物理化学标准,将碱性磷酸酶(EC 3.1.3.1)、α-淀粉酶(EC 3.2.1.1)、γ-谷氨酰转移酶(EC 2.3.2.2)、肌酸激酶(EC 2.7.3.2)、总乳酸脱氢酶(EC 1.1.1.27)和乳酸脱氢酶-1的活性与精液分析结果进行了比较。酶活性取决于睾丸、精囊、前列腺、尿道球腺的功能,并参与生育能力的形成。这些发现有助于阐明精子浆酶的合成作用和某些功能。讨论了上述数据在男性不育治疗以及提高人工授精中精子受精能力方面的实际应用。