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[不同生育能力的射精液蛋白质谱]

[The protein spectrum of ejaculates with different fertilities].

作者信息

Nikolaev A A, Lutskiĭ D L, Lozhkina L V

出版信息

Urol Nefrol (Mosk). 1998 Mar-Apr(2):48-52.

PMID:9577707
Abstract

Immunochemical assay for protein composition was made in 22 fertile and 110 subfertile human sperm samples. Changes in the levels of albumin, prostatic acid phosphatase, sperm-specific tripsin inhibitor, prostate-specific antigen and alpha-2-microglobulin of fertility in spermoplasm were studied in different disturbances of ejaculate fecundating ability. The latter was determined by 14 morphofunctional and physicochemical criteria of sperm fertility. With an increase of the ejaculate volume above 5 ml protein homeostasis showed abnormality. The authors consider functional value of the study proteins in ejaculate, pattern of spermatozoid motor activity, maintenance of normal spermoplasmic pH; discuss practical opportunities of the data obtained for increasing fertility of the sperm used in artificial fertilization, diagnosis and treatment of male infertility.

摘要

对22份正常生育能力和110份生育能力低下的人类精子样本进行了蛋白质组成的免疫化学分析。在射精受精能力的不同紊乱情况下,研究了精子质中白蛋白、前列腺酸性磷酸酶、精子特异性胰蛋白酶抑制剂、前列腺特异性抗原和α-2-微球蛋白水平对生育能力的影响。后者由14项精子生育能力的形态功能和物理化学标准来确定。当射精量增加超过5毫升时,蛋白质稳态显示异常。作者考虑了研究蛋白质在精液中的功能价值、精子运动活性模式、维持正常精子质pH值;讨论了所获数据在提高人工授精所用精子的生育能力、男性不育症诊断和治疗方面的实际应用机会。

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