Shintaku M, Honda T
Department of Pathology, Osaka Red Cross Hospital, Japan.
Histopathology. 1997 Nov;31(5):408-11. doi: 10.1046/j.1365-2559.1997.2870891.x.
To evaluate the immunohistochemistry using an anti-mitochondria antibody in the investigation of various oncocytic lesions of the salivary glands.
Ten cases of adenolymphoma (Warthin's tumour) and one case each of benign oncocytoma and oncocytic carcinoma of the salivary glands were examined. Normal salivary glands were also tested. They were investigated immunohistochemically using mouse monoclonal antibody against human mitochondria. In normal salivary glands, epithelial cells of the striated ducts showed a thick linear immunoreactivity, which corresponded well to the intracytoplasmic distribution pattern of mitochondria. In addition, a small number of swollen epithelial cells showing an intense, finely granular immunoreactivity in the cytoplasm were scattered in the ductal system and acini ('oncocytic metaplasia'). Almost all neoplastic cells involved in adenolymphoma, benign oncocytoma, and oncocytic carcinoma showed an intense, finely granular immunoreactivity in the cytoplasm.
Immunohistochemistry using the anti-mitochondria antibody proved to be a highly sensitive and specific method for light microscopic identification of mitochondria and superior to routine H & E or PTAH stain especially in the detection of isolated oncocytic cells.
评估使用抗线粒体抗体的免疫组织化学方法在涎腺各种嗜酸性细胞病变研究中的应用。
对10例腺淋巴瘤（沃辛瘤）、1例涎腺良性嗜酸性细胞瘤和1例嗜酸性细胞癌进行检查，并检测正常涎腺。使用抗人线粒体小鼠单克隆抗体对其进行免疫组织化学研究。在正常涎腺中，纹状管上皮细胞显示出浓密的线性免疫反应性，这与线粒体的胞质内分布模式相符。此外，在导管系统和腺泡中有少量肿胀的上皮细胞，其胞质内显示出强烈的细颗粒状免疫反应性（“嗜酸性化生”）。几乎所有腺淋巴瘤、良性嗜酸性细胞瘤和嗜酸性细胞癌中的肿瘤细胞胞质内均显示出强烈的细颗粒状免疫反应性。
使用抗线粒体抗体的免疫组织化学方法被证明是一种用于光镜下识别线粒体的高度敏感和特异的方法，尤其在检测孤立的嗜酸性细胞方面优于常规苏木精-伊红染色或磷钨酸苏木精染色。
