Słonina D, Gasińska A
Laboratory of Radiation Biology, Centre of Oncology, Kraków, Poland.
Int J Radiat Biol. 1997 Dec;72(6):693-701. doi: 10.1080/095530097142852.
The purpose of the study was to evaluate the usefulness of the cytokinesis-block micronucleus (MN) assay in assessment of radiosensitivity of lymphocytes in cancer patients. Lymphocytes from 15 cervical cancer patients, 21 head and neck cancer patients, seven lung cancer patients and 19 healthy donors were analysed using MN assay. The proportion of binucleate cells (BC) in cancer patients ranged from 22 to 56% and was significantly lower than in the control group (38-68%). MN frequency assessed five times over 6 months in four healthy donors showed that the interindividual variation was significantly higher than intraindividual. Before (0 Gy) and after irradiation (2 and 4 Gy) no statistical differences in the mean number of MN/BC were observed between healthy donors and cancer patient groups. Nevertheless, statistical cluster analysis allowed each group of donors to be divided into radioresistant and radiosensitive subgroups of patients. They showed significantly different dose response. Separate comparison of the mean MN frequency within all examined radioresistant and radiosensitive subgroups, showed statistically significant differences only after a dose of 4 Gy. At this dose, the lung cancer patients and cervical cancer patients from radiosensitive subgroups presented significantly higher radiosensitivity than the healthy donors. However, healthy donors from radioresistant subgroup did not differ significantly from cancer patients. This work has shown a high variation in interindividual radiosensitivity of donors and suggests the possibility of identifying radiosensitive patients on the basis of MN assay performed on lymphocytes.
本研究的目的是评估胞质分裂阻滞微核（MN）试验在评估癌症患者淋巴细胞放射敏感性方面的实用性。使用MN试验分析了15例宫颈癌患者、21例头颈癌患者、7例肺癌患者和19名健康供者的淋巴细胞。癌症患者双核细胞（BC）的比例在22%至56%之间，显著低于对照组（38%至68%）。对4名健康供者在6个月内进行5次评估的MN频率显示，个体间差异显著高于个体内差异。在照射前（0 Gy）和照射后（2 Gy和4 Gy），健康供者组和癌症患者组之间未观察到MN/BC平均数的统计学差异。然而，统计聚类分析可将每组供者分为放射抗性和放射敏感的患者亚组。它们显示出显著不同的剂量反应。对所有检查的放射抗性和放射敏感亚组内的平均MN频率进行单独比较，仅在4 Gy剂量后显示出统计学显著差异。在此剂量下，放射敏感亚组的肺癌患者和宫颈癌患者的放射敏感性显著高于健康供者。然而，放射抗性亚组的健康供者与癌症患者之间无显著差异。这项工作表明供者个体间放射敏感性存在很大差异，并提示基于淋巴细胞进行MN试验识别放射敏感患者的可能性。
