Cruz-Sánchez J M, Velázquez E, Mateos P F, Velázquez E, Martínez-Molina E
Departamento de Microbiologia y Genética, Universidad de Salmanca, Spain.
Electrophoresis. 1997 Oct;18(11):1909-11. doi: 10.1002/elps.1150181103.
Stable low molecular weight (LMW) RNA comprises molecules used in the taxonomy of microorganisms and in studies on the microbial diversity of populations. However, the use of electrophoretic techniques has been hampered due to the low resolution obtained with techniques used for the separation of this kind of molecule. In this work we develop an electrophoretic method (staircase electrophoresis) that increases the resolution of the technique. This improvement in the resolution adequately resolves the three zones that integrate the profiles of LMW RNA: ribosomic 5S RNA (5S rRNA), class 2 transfer RNA (tRNA), and class 1 transfer RNA, allowing the technique to be applied to taxonomic studies (diagnostic, the identification of the individuals), phylogenetic studies and studies on naturally occurring microbial populations.
稳定的低分子量（LMW）RNA包含用于微生物分类学以及种群微生物多样性研究的分子。然而，由于用于分离这类分子的技术所获得的分辨率较低，电泳技术的应用受到了阻碍。在这项工作中，我们开发了一种电泳方法（阶梯电泳），提高了该技术的分辨率。分辨率的这种提高能够充分分辨构成LMW RNA图谱的三个区域：核糖体5S RNA（5S rRNA）、2类转移RNA（tRNA）和1类转移RNA，从而使该技术能够应用于分类学研究（诊断、个体鉴定）、系统发育研究以及对自然存在的微生物种群的研究。
