Velázquez E, Trujillo M E, Peix A, Palomo J L, García-Benavides P, Mateos P E, Ventosa A, Martínez-Molina E
Departamento de Microbiología y Genética, Universidad de Salamanca, Spain.
Syst Appl Microbiol. 2001 Dec;24(4):490-9. doi: 10.1078/0723-2020-00082.
Low-molecular weight RNA (LMW RNA) analysis using staircase electrophoresis was performed for several species of eukaryotic and prokaryotic microorganisms. According to our results, the LMW RNA profiles of archaea and bacteria contain three zones: 5S RNA, class 1 tRNA and class 2 tRNA. In fungi an additional band is included in the LMW RNA profiles, which correspond to the 5.8S RNA. In archaea and bacteria we found that the 5S rRNA zone is characteristic for each genus and the tRNA profile is characteristic for each species. In eukaryotes the combined 5.8S and 5S rRNA zones are characteristic for each genus and, as in prokaryotes, tRNA profiles are characteristic for each species. Therefore, stable low molecular weight RNA, separated by staircase electrophoresis, can be considered a molecular signature for both prokaryotic and eukaryotic microorganisms. Analysis of the data obtained and construction of the corresponding dendrograms afforded relationships between genera and species; these were essentially the same as those obtained with 16S rRNA sequencing (in prokaryotes) and 18S rRNA sequencing (in eukaryotes).
利用阶梯式电泳对几种真核和原核微生物进行了低分子量RNA（LMW RNA）分析。根据我们的结果，古菌和细菌的LMW RNA谱包含三个区域：5S RNA、1类tRNA和2类tRNA。在真菌中，LMW RNA谱中还包含一条额外的条带，对应于5.8S RNA。在古菌和细菌中，我们发现5S rRNA区域对每个属具有特征性，而tRNA谱对每个物种具有特征性。在真核生物中，5.8S和5S rRNA的组合区域对每个属具有特征性，并且与原核生物一样，tRNA谱对每个物种具有特征性。因此，通过阶梯式电泳分离得到的稳定低分子量RNA可被视为原核和真核微生物的分子特征。对所得数据的分析以及相应树形图的构建揭示了属与种之间的关系；这些关系与通过16S rRNA测序（原核生物）和18S rRNA测序（真核生物）得到的关系基本相同。
