Trujillo M E, Velázquez E, Mateos P F, Martínez-Molina E, Chordi-Corbo A
Departamento de Microbiología y Genética, Universidad de Salamanca, Spain.
Syst Appl Microbiol. 2001 Jul;24(2):290-3. doi: 10.1078/0723-2020-00030.
Staircase electrophoresis (SCE) in polyacrilamide gels was used to analyze the stable low-molecular weight (LMW) RNA profiles of several propane and butane oxidizing bacteria belonging to different species and genera. Differences in the number and distribution of the RNA bands in these profiles allowed us to differentiate among them. Congruent results were found between the established classification of these bacteria and results obtained by LMW RNA profiling and moreover, some misclassified strains can be assigned to the correct genus and species using this technique. LMW RNA profiling by staircase electrophoresis, which makes possible the analysis of a large number of strains in a short time, permits rapid identification of hydrocarbon metabolizing species when compared with LMW RNA profiles of reference strains.
采用聚丙烯酰胺凝胶中的阶梯式电泳（SCE）分析了几种属于不同种属的丙烷和丁烷氧化细菌的稳定低分子量（LMW）RNA图谱。这些图谱中RNA条带数量和分布的差异使我们能够区分它们。这些细菌的既定分类与通过LMW RNA谱分析获得的结果一致，此外，使用该技术可以将一些分类错误的菌株归为正确的属和种。与参考菌株的LMW RNA谱相比，阶梯式电泳的LMW RNA谱分析能够在短时间内分析大量菌株，从而可以快速鉴定烃代谢物种。
