Daba S, Mansour N S, Youssef F G, Shanbaky N M, el Sawaf B M
U.S. Naval Medical Research Unit No. 3, Cairo, Egypt.
J Egypt Soc Parasitol. 1997 Dec;27(3):795-804.
Fifty five protein bands with relative mobilities of 8,954 to 245,471 kilo Daltons (kD) were electrophoretically separated from 12 feeding media of blood from 4 natural vertebrate hosts of Phlebotomus langeroni. The feeding media included human, dog (Canis familiaris), rat (Rattus rattus) and turkey (Melagris gallopava) bloods without or with Leishmania infantum or L. major promastigotes. Protein bands were identical among the feeding media of one host's blood but varied in number (24-28 bands) and relative mobilities among the various hosts' blood. Some protein fractions were common among the various hosts blood, others were only present in two or three hosts' blood and some were restricted to one host blood and were unique for each host. This study provides data which may help in understanding why blood from different natural hosts may variably influence the life cycle of Leishmania parasite in the sand fly gut.
从朗氏白蛉的4种天然脊椎动物宿主的12份血液喂食培养基中，通过电泳分离出了55条相对迁移率在8,954至245,471千道尔顿（kD）之间的蛋白条带。这些喂食培养基包括不含或含有婴儿利什曼原虫或硕大利什曼原虫前鞭毛体的人血、狗血（犬属）、鼠血（褐家鼠）和火鸡血（原鸡）。同一宿主血液的喂食培养基中的蛋白条带是相同的，但不同宿主血液中的蛋白条带数量（24 - 28条）和相对迁移率有所不同。不同宿主血液中有一些蛋白组分是共同的，其他一些仅存在于两三种宿主血液中，还有一些则局限于一种宿主血液中，且每种宿主都有其独特性。本研究提供的数据可能有助于理解为什么来自不同天然宿主的血液会对利什曼原虫在白蛉肠道中的生命周期产生不同影响。
