Montoya Liliana, Gállego Montserrat, Gavignet Béatrice, Piarroux Renaud, Rioux Jean-Antoine, Portús Montserrat, Fisa Roser
Laboratory of Parasitology, Universitat de Barcelona, Barcelona, Spain.
Am J Trop Med Hyg. 2007 May;76(5):888-95.
Leishmania infantum polymorphism was studied by DNA microsatellite analysis of 110 L. infantum stocks (94 from dogs, 15 from sand flies, and 1 from a human visceral case) from a rural leishmaniasis-endemic area (Priorat) in northeastern Spain. Three microsatellites of the eight present in three fragments (internal transcribed spacer, Lm4, and Lm2) of L. infantum nuclear DNA are polymorphic inside the focus, resulting in 17 genotypes. Isolates from dogs and sand flies had different allelic compositions and shared only four genotypes. Microsatellite analysis is useful for L. infantum genotyping and epidemiologic tracking. Its application with strains from dogs and vectors in an area endemic for leishmaniasis shows the heterogeneous distribution of L. infantum in hosts living in sympatric conditions.
通过对来自西班牙东北部利什曼病流行农村地区（普里奥拉托）的110株婴儿利什曼原虫（94株来自犬类，15株来自白蛉，1株来自人类内脏病例）进行DNA微卫星分析，研究了婴儿利什曼原虫的多态性。婴儿利什曼原虫核DNA三个片段（内转录间隔区、Lm4和Lm2）中的八个微卫星中的三个在疫区内具有多态性，产生了17种基因型。来自犬类和白蛉的分离株具有不同的等位基因组成，仅共享四种基因型。微卫星分析对于婴儿利什曼原虫基因分型和流行病学追踪很有用。它在利什曼病流行地区与犬类和媒介菌株的应用表明，婴儿利什曼原虫在同域生活的宿主中分布不均。
