De Zeeuw C I, Hoogenraad C C, Goedknegt E, Hertzberg E, Neubauer A, Grosveld F, Galjart N
Department of Anatomy, Erasmus University Rotterdam, The Netherlands.
Neuron. 1997 Dec;19(6):1187-99. doi: 10.1016/s0896-6273(00)80411-0.
Intracellular localization of organelles may depend in part on specific cytoplasmic linker proteins (CLIPs) that link membranous organelles to microtubules. Here, we characterize rat cDNAs encoding a novel, brain-specific CLIP of 115 kDa. This protein contains two N-terminal microtubule-binding domains and a long coiled-coil region; it binds to microtubules and is homologous to CLIP-170, a protein mediating the binding of endosomes to microtubules. CLIP-115 is enriched in the dendritic lamellar body (DLB), a recently discovered organelle predominantly present in bulbous dendritic appendages of neurons linked by dendrodendritic gap junctions. Local microtubule depolymerization leads to a temporary reduction of DLBs. These results suggest that CLIP-115 operates in the control of brain-specific organelle translocations.
细胞器的细胞内定位可能部分取决于特定的细胞质连接蛋白（CLIPs），这些蛋白将膜性细胞器与微管相连。在此，我们鉴定了编码一种新型的、大脑特异性的115 kDa CLIP的大鼠cDNA。该蛋白包含两个N端微管结合结构域和一个长的卷曲螺旋区域；它与微管结合，并且与CLIP-170同源，CLIP-170是一种介导内体与微管结合的蛋白。CLIP-115在树突状层状体（DLB）中富集，DLB是最近发现的一种细胞器，主要存在于通过树突-树突缝隙连接相连的神经元的球状树突附属物中。局部微管解聚导致DLB暂时减少。这些结果表明CLIP-115在大脑特异性细胞器转运的控制中发挥作用。
