Demaimay F, Dazord L, Roucoux A, Noiret N, Patin H, Moisan A
Laboratoire de Chimie Organique et des Substances Naturelles, CNRS ESA 6052, Ecole Nationale Supérieure de Chimie de Rennes, France.
Nucl Med Biol. 1997 Nov;24(8):701-5. doi: 10.1016/s0969-8051(97)00096-6.
In this study, we have investigated the preparation of rhenium-188 nitridobis(N-ethoxy-N-ethyldithiocarbamate) [188ReN(NOET)2] (NOET = Et(EtO)NCS2), analogous to the known technetium-99m radiopharmaceutical. The new 188Re complex was synthesized in good yield with a satisfactory radiochemical purity, using a kit method. The subcellular localization of both radiopharmaceuticals in granulocytes was observed by microautoradiography. The uptake was independent of the radionuclide and predominantly nuclear. Furthermore, HPLC was used to characterize the 99mTc complex before and after blood cell labelling and revealed that the intact radiopharmaceutical was involved.
在本研究中，我们研究了铼-188氮双（N-乙氧基-N-乙基二硫代氨基甲酸盐）[188ReN(NOET)2]（NOET = Et(EtO)NCS2）的制备方法，该化合物类似于已知的锝-99m放射性药物。采用试剂盒法以良好的产率合成了新的188Re配合物，其放射化学纯度令人满意。通过显微放射自显影观察了两种放射性药物在粒细胞中的亚细胞定位。摄取与放射性核素无关，主要定位于细胞核。此外，利用高效液相色谱法对血细胞标记前后的99mTc配合物进行了表征，结果表明完整的放射性药物参与了标记过程。
