Li H, Snowder G, O'Toole D, Crawford T B
Animal Diseases Research Unit, U.S. Department of Agriculture-Agricultural Research Service, Pullman, Washington 99164, USA.
J Clin Microbiol. 1998 Jan;36(1):223-6. doi: 10.1128/JCM.36.1.223-226.1998.
The pattern of acquisition of ovine herpesvirus 2 (OHV-2) infection in lambs was examined by a competitive-inhibition enzyme-linked immunosorbent assay and PCR. Newborn lambs (n = 118) did not exhibit antibody at birth. Viral DNA in peripheral blood leukocytes was detected in only 3% (n = 77) of newborn lambs before suckling. After nursing, viral DNA was sporadically present in about 10 to 20% of lambs until about 3 months of age. Thereafter, strong DNA signals began to appear in increasing numbers of lambs, reaching 100% by 5.5 months of age. Viral DNA in nasal secretions began to be detectable in about 30% of lambs at 5.5 months of age, achieved significant levels in 88% of lambs by 7.5 months of age, and then declined. The kinetics of the humoral immune response in lambs paralleled those of viral DNA in nasal secretions but did not parallel its presence in blood leukocytes. In the experiment to define the time of infection of OHV-2 in lambs, all five lambs separated from the flock at 2.5 months of age remained uninfected until the termination of the experiment at 1 year of age. In contrast, lambs weaned at 2.5 months of age and returned to the flock had become infected at 3.5 months of age. Weaning and separation from the flock at 3.5 months of age did not prevent infection. The study showed that OHV-2 infection does not commonly occur in perinatal lambs and that OHV-2-free sheep can be established by separation of lambs at the proper time, which has important implications for potential control measures.
通过竞争抑制酶联免疫吸附测定和聚合酶链反应（PCR）检测了羔羊感染绵羊疱疹病毒2型（OHV-2）的模式。新生羔羊（n = 118）出生时未表现出抗体。仅3%（n = 77）的新生羔羊在吮乳前外周血白细胞中检测到病毒DNA。哺乳后，病毒DNA偶尔出现在约10%至20%的羔羊中，直至约3月龄。此后，越来越多的羔羊开始出现强烈的DNA信号，到5.5月龄时达到100%。5.5月龄时，约30%的羔羊鼻分泌物中开始可检测到病毒DNA，7.5月龄时88%的羔羊鼻分泌物中病毒DNA达到显著水平，然后下降。羔羊体液免疫反应的动力学与鼻分泌物中病毒DNA的动力学平行，但与血液白细胞中病毒DNA的存在情况不平行。在确定羔羊感染OHV-2时间的实验中，所有5只在2.5月龄时与羊群隔离的羔羊直到1岁实验结束时仍未感染。相比之下，2.5月龄断奶并返回羊群的羔羊在3.5月龄时已被感染。3.5月龄断奶并与羊群隔离并不能预防感染。该研究表明，OHV-2感染在围产期羔羊中并不常见，通过在适当时间隔离羔羊可以建立无OHV-2的羊群，这对潜在的控制措施具有重要意义。
