Akihisa T, Yasukawa K, Kimura Y, Takase S, Yamanouchi S, Tamura T
College of Science and Technology, Nihon University, Tokyo, Japan.
Chem Pharm Bull (Tokyo). 1997 Dec;45(12):2016-23. doi: 10.1248/cpb.45.2016.
The nonsaponifiable lipids of camellia and sasanqua oils from the seeds of Camellia japonica L. and C. sasanqua THUNB., respectively, were investigated for their triterpene alcohol constituents. This led to the isolation of twenty-seven triterpene alcohols of which seven were novel naturally occurring compounds, tirucalla-5,7,24-trien-3 beta-ol (1), lemmaphylla-7,21-dien-3 beta-ol (2), isoeuphol (3), isotirucallol (4), (24R)-24,25-epoxybutyrospermol (5) and its 24S-epimer (6), and isoaglaiol (7). The structures were determined by spectroscopic and chemical methods. The inhibitory effects of 3, 4 a mixture of 5 and 6, a mixture of 7 and its 24S-epimer (aglaiol), and eight known triterpene alcohols isolated in this study were evaluated in ear inflammation in mice induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). The 50% inhibitory dose of these triterpenes for TPA-induced inflammation (1 microgram per ear) was 0.2-0.9 mg/ear.
分别对来自日本山茶（Camellia japonica L.）种子的山茶油和茶梅（C. sasanqua THUNB.）种子的茶梅油中的不皂化脂质的三萜醇成分进行了研究。由此分离出27种三萜醇，其中7种是新的天然化合物，即香树脂醇-5,7,24-三烯-3β-醇（1）、瘤蕨叶醇-7,21-二烯-3β-醇（2）、异大戟醇（3）、异香树脂醇（4）、（24R）-24,25-环氧丁酰基香树精醇（5）及其24S-差向异构体（6）和异蛇麻脂醇（7）。通过光谱和化学方法确定了其结构。在由12-O-十四烷酰佛波醇-13-乙酸酯（TPA）诱导的小鼠耳部炎症中，评估了3、4、5和6的混合物、7及其24S-差向异构体（蛇麻脂醇）的混合物以及本研究中分离出的8种已知三萜醇的抑制作用。这些三萜对TPA诱导的炎症（每耳1微克）的50%抑制剂量为0.2-0.9毫克/耳。
