Berman N S, Siegel S E, Nachum R, Lipsey A, Leedom J
J Pediatr. 1976 Apr;88(4 Pt 1):553-6. doi: 10.1016/s0022-3476(76)80004-2.
The limulus lysate assay was utilized to detect and quantitate endotoxin in cerebrospinal fluid from 232 patients with suspected meningitis. The assay was positive in initial specimens of CSF from all 86 patients with gram-negative bacterial meningitis and was uniformly negative in the remaining 146 patients with a variety of infectious and noninfectious processes. Endotoxin concentrations in initial specimens of CSF from patients with gram-negative meningitis ranged from 4 to 2,000 ng/ml. No correlation between initial CSF levels of endotoxin and initial clinical or laboratory variables of infection was noted. With antibiotic therapy, CSF concentrations of endotoxin fall rapidly to undetectable levels after five days.
采用鲎试剂法检测并定量分析了232例疑似脑膜炎患者脑脊液中的内毒素。在所有86例革兰阴性菌性脑膜炎患者的脑脊液初始标本中，该检测呈阳性，而在其余146例患有各种感染性和非感染性疾病的患者中均呈阴性。革兰阴性菌性脑膜炎患者脑脊液初始标本中的内毒素浓度为4至2000 ng/ml。未发现脑脊液内毒素初始水平与感染的初始临床或实验室指标之间存在相关性。使用抗生素治疗后，五天内脑脊液内毒素浓度迅速降至检测不到的水平。
