基于通过聚合酶链式反应（PCR）检测到的DNA来测定空肠弯曲菌分离株对水中煮沸的敏感性进行鉴别。
Differentiation of Campylobacter isolates on the basis of sensitivity to boiling in water as measured by PCR-detectable DNA.
作者信息
Mohran Z S, Arthur R R, Oyofo B A, Peruski L F, Wasfy M O, Ismail T F, Murphy J R
机构信息
U.S. Naval Medical Research Unit No. 3, Cairo, Egypt.
出版信息
Appl Environ Microbiol. 1998 Jan;64(1):363-5. doi: 10.1128/AEM.64.1.363-365.1998.
Abstract
Differential sensitivity for the release of PCR-detectable genomic DNA upon boiling in water is reported for 45 Campylobacter jejuni and Campylobacter coli strains isolated in Egypt. All of the strains released PCR-detectable DNA when treated with proteinase K and sodium dodecyl sulfate. When DNA was extracted from these strains by boiling in water, nine (20%) of the strains were PCR negative or resistant to boiling, suggesting the presence of boiling-sensitive and boiling-resistant phenotypes.
摘要
据报道，从埃及分离出的45株空肠弯曲菌和结肠弯曲菌菌株在沸水中煮沸后释放可通过聚合酶链反应（PCR）检测的基因组DNA的敏感性存在差异。所有菌株在用蛋白酶K和十二烷基硫酸钠处理后均释放出可通过PCR检测的DNA。当通过在水中煮沸从这些菌株中提取DNA时，有9株（20%）菌株PCR检测呈阴性或对煮沸有抗性，这表明存在对煮沸敏感和对煮沸有抗性的表型。
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