Kamachi S, Sekimoto H, Kondo N, Sakai S
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
Plant Cell Physiol. 1997 Nov;38(11):1197-206. doi: 10.1093/oxfordjournals.pcp.a029106.
In cucumber (Cucumis sativus L.) plants, it has been reported that a high correlation existed between the evolution of ethylene from the apices and the development of female flowers. We isolated three distinct cDNA encoding 1-aminocyclopropane-1-carboxylate (ACC) synthase from cucumber. Among of these, only CS-ACS2 mRNA was detected at the apices of gynoecious cucumber in which female flowers were developing. The expression of the CS-ACS2 gene was examined in the apices of three cucumber cultivars (Rensei, Ougonmegami 2-gou and Shimoshirazu) by RNA gel blot analysis. In these cultivars, both the timing and the levels of expression of the CS-ACS2 transcript were correlated with the development of female flowers at the nodes. Furthermore, the timing of the induction of expression of the CS-ACS2 gene at the apex corresponded to that of the action of ethylene in induction of the first female flower at the apex of gynoecious cucumber plants. These results suggest that the development of female flowers might be regulated by the level of CS-ACS2 mRNA at the apex.
在黄瓜（Cucumis sativus L.）植株中，据报道，茎尖乙烯释放量的变化与雌花的发育之间存在高度相关性。我们从黄瓜中分离出三个不同的编码1-氨基环丙烷-1-羧酸（ACC）合酶的cDNA。其中，仅在发育雌花的全雌黄瓜茎尖检测到CS-ACS2 mRNA。通过RNA凝胶印迹分析检测了三个黄瓜品种（仁生、黄金女神2号和下白）茎尖中CS-ACS2基因的表达。在这些品种中，CS-ACS2转录本的表达时间和水平均与节上雌花的发育相关。此外，茎尖CS-ACS2基因表达诱导的时间与乙烯诱导全雌黄瓜植株茎尖第一朵雌花的作用时间一致。这些结果表明，雌花的发育可能受茎尖CS-ACS2 mRNA水平的调控。
