Quade M J, Roth J A
Department of Microbiology, Immunology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
Vet Immunol Immunopathol. 1997 Sep 19;58(3-4):239-48. doi: 10.1016/s0165-2427(97)00048-2.
A direct, rapid, quantitative colorimetric assay to determine neutrophil primary granule degranulation was adapted for use with bovine neutrophils. The assay measures the exocytosis of myeloperoxidase (MPO) using 3,3',5,5'-tetramethylbenzidine as a substrate. The assay was validated by evaluating the effects of various stimulants and inhibitors of degranulation, the kinetics of primary granule exocytosis, and comparing the total myeloperoxidase content of neutrophils obtained from calves and adults. The results demonstrate that the assay is capable of detecting important differences that may occur in degranulation of bovine neutrophil primary granules and in total neutrophil myeloperoxidase content.
一种用于测定中性粒细胞初级颗粒脱颗粒的直接、快速、定量比色测定法被改编用于牛中性粒细胞。该测定法使用3,3',5,5'-四甲基联苯胺作为底物来测量髓过氧化物酶（MPO）的胞吐作用。通过评估各种脱颗粒刺激剂和抑制剂的作用、初级颗粒胞吐作用的动力学以及比较从犊牛和成年牛获得的中性粒细胞的总髓过氧化物酶含量，对该测定法进行了验证。结果表明，该测定法能够检测牛中性粒细胞初级颗粒脱颗粒以及中性粒细胞总髓过氧化物酶含量可能出现的重要差异。
