Röhrdanz E, Kahl R
Institute of Toxicology, Heinrich-Heine-Universität Düsseldorf, Germany.
Free Radic Biol Med. 1998 Jan 1;24(1):27-38. doi: 10.1016/s0891-5849(97)00159-7.
The effect of hydrogen peroxide (H2O2) on the expression of different antioxidant enzymes was investigated in primary rat hepatocytes and the rat hepatoma H4IIE cell line. Catalase mRNA expression and enzyme activity decreased during rat hepatocyte culture. Exposure of hepatocytes to H2O2 prevented this decrease in catalase mRNA expression, catalase expression was induced 2-fold. MnSOD message levels showed a peak after 12 h of culture and MnSOD enzyme activity increased similarly. MnSOD mRNA expression was also induced after exposure to H2O2. Cu/ZnSOD mRNA expression remained constant during culturing and was not affected by H2O2 treatment. In confluent hepatoma H4IIE cells catalase mRNA expression was lower than in early hepatocyte cultures and could be induced 2-fold upon treatment with H2O2. Actinomycin D alone caused the same amount of induction of catalase mRNA in rat hepatocytes as in combination with H2O2. Exposure of hepatocytes to cycloheximide did not influence the induction of catalase mRNA by H2O2. In rat hepatoma H4IIE cells the induction of catalase mRNA by H2O2 was prevented by the addition of actinomycin D or cycloheximide. Although induction of catalase mRNA by H2O2 was found in rat hepatocytes and H4IIE cells, gene expression of catalase does not appear to be regulated in both cell types in the same manner.
在原代大鼠肝细胞和大鼠肝癌H4IIE细胞系中研究了过氧化氢（H2O2）对不同抗氧化酶表达的影响。在大鼠肝细胞培养过程中，过氧化氢酶mRNA表达和酶活性降低。将肝细胞暴露于H2O2可防止过氧化氢酶mRNA表达的这种降低，过氧化氢酶表达诱导增加了2倍。锰超氧化物歧化酶（MnSOD）信息水平在培养12小时后出现峰值，MnSOD酶活性也有类似增加。暴露于H2O2后，MnSOD mRNA表达也被诱导。铜/锌超氧化物歧化酶（Cu/ZnSOD）mRNA表达在培养过程中保持恒定，且不受H2O2处理的影响。在汇合的肝癌H4IIE细胞中，过氧化氢酶mRNA表达低于早期肝细胞培养物，经H2O2处理后可诱导增加2倍。单独使用放线菌素D在大鼠肝细胞中引起的过氧化氢酶mRNA诱导量与与H2O2联合使用时相同。将肝细胞暴露于放线菌酮并不影响H2O2对过氧化氢酶mRNA的诱导。在大鼠肝癌H4IIE细胞中，添加放线菌素D或放线菌酮可阻止H2O2对过氧化氢酶mRNA的诱导。虽然在大鼠肝细胞和H4IIE细胞中都发现了H2O2对过氧化氢酶mRNA的诱导，但过氧化氢酶的基因表达在两种细胞类型中似乎不是以相同方式调节的。
