Tsuzaka K, Fujii T, Akizuki M, Mimori T, Tojo T, Fujii H, Tsukatani Y, Kubo A, Homma M
Arthritis Rheum. 1994 Jan;37(1):88-92. doi: 10.1002/art.1780370113.
To evaluate the clinical significance of antibodies to native or denatured (anti-n or anti-d) 60- or 52-kd Ro/SS-A proteins (60K or 52K) in Sjögren's syndrome (SS).
The presence of antibodies to denatured and native Ro/SS-A proteins was determined by immunoblotting and immunoprecipitation, respectively. Salivary gland dysfunction was evaluated by salivary function scintigraphy.
The incidence of anti-d-60K without anti-d-52K was lower among patients with systemic lupus erythematosus with SS (SLE/SS) and among those with primary SS, compared with patients who had SLE without SS, whereas anti-d-52K without anti-d-60K was more common in SLE/SS patients and primary SS patients than in SLE patients without SS. All of the patients with anti-Ro/SS-A had anti-n-60K. Serologic abnormalities and salivary gland dysfunction were associated with anti-n-60K in SS, whereas Hashimoto's thyroiditis in SS was related to anti-d-60K. Anti-d-52K was not associated with any extraglandular or glandular symptoms in SS.
The data indicate that anti-n-60K, which appears to recognize conformational epitopes, is associated with clinical features of SS characterized by glandular dysfunction.
评估抗天然或变性（抗n或抗d）60kd或52kd Ro/SS-A蛋白（60K或52K）抗体在干燥综合征（SS）中的临床意义。
分别通过免疫印迹法和免疫沉淀法检测抗变性和抗天然Ro/SS-A蛋白的存在情况。通过唾液功能闪烁显像评估唾液腺功能障碍。
与无SS的系统性红斑狼疮（SLE）患者相比，合并SS的SLE（SLE/SS）患者和原发性SS患者中，仅抗d-60K而无抗d-52K的发生率较低，而仅抗d-52K而无抗d-60K在SLE/SS患者和原发性SS患者中比无SS的SLE患者更常见。所有抗Ro/SS-A患者均有抗n-60K。血清学异常和唾液腺功能障碍与SS中的抗n-60K相关，而SS中的桥本甲状腺炎与抗d-60K相关。抗d-52K与SS中的任何腺外或腺体症状均无关。
数据表明，似乎识别构象表位的抗n-60K与以腺体功能障碍为特征的SS临床特征相关。
