Bielaszewska M, Janda J, Bláhová K, Minaríková H, Jíková E, Karmali M A, Laubová J, Sikulová J, Preston M A, Khakhria R, Karch H, Klazarová H, Nyc O
Institute of Medical Microbiology, The 2nd Medical Faculty, Charles University, Prague, Czech Republic.
Epidemiol Infect. 1997 Dec;119(3):299-305. doi: 10.1017/s0950268897008297.
A cluster of four cases of haemolytic uraemic syndrome in children occurred in Northern Bohemia, Czech Republic, between 15 June and 7 July, 1995. All the cases had significantly elevated titres of anti-O157 lipopolysaccharide (LPS) antibodies as detected by the indirect haemagglutination assay. All but one of them had drunk unpasteurized goat's milk from the same farm within the week before the disease. Evidence of E. coli O157 infection was subsequently found in 5 of 15 regular drinkers of the farm's raw goat's milk; four of them were asymptomatic, 1 had mild diarrhoea at the end of June. Verocytotoxin 2-producing E. coli O157:H7 strains of phage type 2 and of identical pulsed-field gel electrophoresis patterns were isolated from 1 of 2 farm goats and from 1 of the asymptomatic goat's milk drinkers. The frequency of anti-O157 LPS antibodies found among regular drinkers of the farm's raw goat's milk (33%; 5 of 15) was significantly higher than that found in control population (0%; none of 45) (P = 0.0005; Fisher's exact test). Our findings indicate that goats may be a reservoir of E. coli O157:H7 and a source of the infection for humans; raw goat's milk may serve as a vehicle of the pathogen transmission.
1995年6月15日至7月7日期间，捷克共和国北波希米亚地区出现了4例儿童溶血性尿毒症综合征病例。通过间接血凝试验检测发现，所有病例的抗O157脂多糖（LPS）抗体滴度均显著升高。除1例病例外，其他病例在发病前一周内均饮用了来自同一农场的未经巴氏消毒的山羊奶。随后，在该农场生山羊奶的15名经常饮用者中，有5人被检测出感染了大肠杆菌O157；其中4人无症状，1人在6月底出现轻度腹泻。从2只农场山羊中的1只以及1名无症状的山羊奶饮用者体内，分离出了噬菌体分型为2型且脉冲场凝胶电泳图谱相同的产志贺毒素2的大肠杆菌O157:H7菌株。该农场生山羊奶经常饮用者中抗O157 LPS抗体的检出率（33%，15人中有5人）显著高于对照组人群（0%，45人中有0人）（P = 0.0005；Fisher精确检验）。我们的研究结果表明，山羊可能是大肠杆菌O157:H7的宿主，也是人类感染的来源；生山羊奶可能是病原体传播的载体。
