Ramot B, Brok-Simoni F, Chweidan E, Ashkenazi Y E
Br J Haematol. 1976 Sep;34(1):79-85. doi: 10.1111/j.1365-2141.1976.tb00176.x.
The activities of glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate: NADP oxidoreductase, G6PD), 6 phosphate glucono dehydrogenase (6 phospho-D-gluconate: NADP oxidoreductase, 6PGD) lactate dehydrogenase (D-lactate: NAD oxidoreductase, LDH), glutamate oxaloacetate transaminase (L-aspartate: 2-oxo-glutarate aminotransferase, GOT) and hexokinase (ATP: D-hexo-6-phosphotrans-ferase, Hx) were measured over 24 h in isolated lymphocytes of normal subjects and in white cells of patients with chronic lymphatic leukaemia (CLL). The activitty patterns of all enzymes in the normal lymphocytes were similar. A computed pattern of all the results exhibited a circadian rhythm of activity with the highest level at 16.00 hours. The oscillations in the activities of the same enzymes in the CLL cells differed among the patients, although all the enzymes of the same individual showed a similar diurnal rhythmic pattern. All peaks in this group appeared between 20.00 and 08.00 hours. The possible importance of these observations in setting up therapeutic schedules was raised.
在正常受试者的分离淋巴细胞以及慢性淋巴细胞白血病（CLL）患者的白细胞中，对葡萄糖-6-磷酸脱氢酶（D-葡萄糖-6-磷酸：NADP氧化还原酶，G6PD）、6-磷酸葡萄糖酸脱氢酶（6-磷酸-D-葡萄糖酸：NADP氧化还原酶，6PGD）、乳酸脱氢酶（D-乳酸：NAD氧化还原酶，LDH）、谷氨酸草酰乙酸转氨酶（L-天冬氨酸：2-氧代戊二酸氨基转移酶，GOT）和己糖激酶（ATP：D-己糖-6-磷酸转移酶，Hx）的活性进行了24小时的测定。正常淋巴细胞中所有酶的活性模式相似。所有结果的计算模式显示出活性的昼夜节律，在16:00时水平最高。CLL细胞中相同酶活性的振荡在患者之间有所不同，尽管同一个体的所有酶都表现出相似的昼夜节律模式。该组中的所有峰值出现在20:00至08:00之间。这些观察结果在制定治疗方案中的潜在重要性被提了出来。
