Ferretti M, Gionchetti P, Rizzello F, Venturi A, Stella P, Corti F, Mizrahi J, Miglioli M, Campieri M
1a Clinica Medica e Gastroenterologia Università di Bologna, Policlinico Sant' Orsola, Italy.
Dig Dis Sci. 1997 Dec;42(12):2606-11. doi: 10.1023/a:1018897519880.
Nitric oxide is thought to play an important role in modulating the inflammatory process. Recently an increase in the inducible form of nitric oxide synthase (iNOS) has been found in the rat trinitrobenzene sulfonic acid model of experimental colitis, and inhibition of nitric oxide synthase activity resulted in an amelioration of tissue injury. The aim of our study was to evaluate in vivo intracolonic release of nitric oxide in this model of colitis. Experimental colitis was induced in male Sprague-Dawley rats by a single intracolonic administration of trinitrobenzene sulfonic acid. Nitrite levels were determined in rectal dialysates by HPLC. The tissue myeloperoxidase and iNOS and the luminal leukotriene B4 were also measured. Nitrite levels were significantly increased in rectal dialysates during colitis and correlated significantly with tissue myeloperoxidase and iNOS activity. The correlation between nitrite dialysate levels and wall iNOS activity confirms that nitrite in dialysates is produced by inflammatory cells and not by colonic bacterial flora. Determination of nitrite levels in rectal dialysates seems a valuable method to monitor colonic inflammation in rat trinitrobenzene sulfonic acid colitis.
一氧化氮被认为在调节炎症过程中发挥重要作用。最近，在大鼠三硝基苯磺酸实验性结肠炎模型中发现诱导型一氧化氮合酶（iNOS）增加，抑制一氧化氮合酶活性可改善组织损伤。我们研究的目的是评估该结肠炎模型中结肠内一氧化氮的体内释放情况。通过单次结肠内给予三硝基苯磺酸在雄性Sprague-Dawley大鼠中诱导实验性结肠炎。用高效液相色谱法测定直肠透析液中的亚硝酸盐水平。还测量了组织髓过氧化物酶、iNOS和肠腔白三烯B4。结肠炎期间直肠透析液中的亚硝酸盐水平显著升高，且与组织髓过氧化物酶和iNOS活性显著相关。透析液中亚硝酸盐水平与肠壁iNOS活性之间的相关性证实，透析液中的亚硝酸盐是由炎症细胞产生的，而非结肠细菌菌群。测定直肠透析液中的亚硝酸盐水平似乎是监测大鼠三硝基苯磺酸结肠炎中结肠炎症的一种有价值的方法。
