Gálvez J, Coelho G, Crespo M E, Cruz T, Rodríguez-Cabezas M E, Concha A, Gonzalez M, Zarzuelo A
Department of Pharmacology, School of Pharmacy, University of Granada, Granada, Spain.
Aliment Pharmacol Ther. 2001 Dec;15(12):2027-39. doi: 10.1046/j.1365-2036.2001.01133.x.
Morin, a bioflavonoid with antioxidant properties, shows intestinal anti-inflammatory activity in the acute phase of the trinitrobenzenesulphonic acid model of rat colitis.
To assess the anti-inflammatory activity of morin in the chronic stages of trinitrobenzenesulphonic acid-induced rat colitis.
Rats were rendered colitic by a single colonic instillation of 30 mg of the hapten trinitrobenzenesulphonic acid dissolved in 0.25 mL of 50% ethanol. A group of colitic animals was given morin orally at doses of 25 mg/kg daily. Animals were sacrificed every week for 4 weeks. Colonic damage was evaluated macroscopically and microscopically. Different biochemical markers of colonic inflammation were also assayed, including myeloperoxidase activity, leukotriene B4 and interleukin-1beta synthesis, glutathione and malonyldialdehyde levels and nitric oxide synthase activity.
The administration of morin facilitated tissue recovery during the 4 weeks following colonic insult with trinitrobenzenesulphonic acid, as demonstrated macroscopically and microscopically, as well as biochemically by a reduction in myeloperoxidase activity. The intestinal anti-inflammatory effect of morin was accompanied by a significant reduction in colonic leukotriene B4 and interleukin-1beta levels, improvement in colonic oxidative stress and inhibition of colonic nitric oxide synthase activity.
Morin exerts a beneficial anti-inflammatory effect in the chronic phase of trinitrobenzenesulphonic acid-induced rat colitis through the down-regulation of some of the mediators involved in the intestinal inflammatory response, including free radicals, cytokines, leukotriene B4 and nitric oxide.
桑色素是一种具有抗氧化特性的生物类黄酮，在三硝基苯磺酸大鼠结肠炎模型的急性期显示出肠道抗炎活性。
评估桑色素在三硝基苯磺酸诱导的大鼠结肠炎慢性期的抗炎活性。
通过向大鼠结肠内单次灌注溶解于0.25 mL 50%乙醇中的30 mg半抗原三硝基苯磺酸使大鼠患结肠炎。一组患结肠炎的动物每天口服25 mg/kg的桑色素。连续4周每周处死动物。对结肠损伤进行宏观和微观评估。还检测了结肠炎症的不同生化标志物，包括髓过氧化物酶活性、白三烯B4和白细胞介素-1β合成、谷胱甘肽和丙二醛水平以及一氧化氮合酶活性。
桑色素给药促进了三硝基苯磺酸结肠损伤后4周内的组织恢复，这在宏观、微观以及生化方面均有体现，即髓过氧化物酶活性降低。桑色素的肠道抗炎作用伴随着结肠白三烯B4和白细胞介素-1β水平的显著降低、结肠氧化应激的改善以及结肠一氧化氮合酶活性的抑制。
桑色素通过下调一些参与肠道炎症反应的介质，包括自由基、细胞因子、白三烯B4和一氧化氮等，在三硝基苯磺酸诱导的大鼠结肠炎慢性期发挥有益的抗炎作用。
