Mellersh C S, Langston A A, Acland G M, Fleming M A, Ray K, Wiegand N A, Francisco L V, Gibbs M, Aguirre G D, Ostrander E A
Clinical Research Division-M318, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.
Genomics. 1997 Dec 15;46(3):326-36. doi: 10.1006/geno.1997.5098.
A genetic linkage map of the canine genome has been developed by typing 150 microsatellite markers using 17 three-generation pedigrees, composed of 163 F2 individuals. One hundred and thirty-nine markers were linked to at least one other marker with a lod score > or = 3.0, identifying 30 linkage groups. The largest chromosome had 9 markers spanning 106.1 cM. The average distance between markers was 14.03 cM, and the map covers an estimated 2073 cM. Eleven markers were informative on the mapping panel, but were unlinked to any other marker. These likely represent single markers located on small, distinct canine chromosomes. This map will be the initial resource for mapping canine traits of interest and serve as a foundation for development of a comprehensive canine genetic map.
利用17个三代家系（由163个F2个体组成）对150个微卫星标记进行分型，构建了犬基因组的遗传连锁图谱。139个标记与至少一个其他标记连锁，其lod分数≥3.0，确定了30个连锁群。最大的染色体有9个标记，跨度为106.1厘摩。标记之间的平均距离为14.03厘摩，该图谱估计覆盖2073厘摩。11个标记在定位板上提供了信息，但未与任何其他标记连锁。这些可能代表位于小的、不同的犬染色体上的单个标记。该图谱将作为定位感兴趣的犬性状的初始资源，并为构建全面的犬遗传图谱奠定基础。
