Feldmeyer B, Pen I, Beukeboom L W
Theoretical Biology, Centre for Ecological and Evolutionary Studies, University of Groningen, the Netherlands.
Insect Mol Biol. 2010 Aug;19(4):575-81. doi: 10.1111/j.1365-2583.2010.01016.x. Epub 2010 May 20.
We present the first molecular marker linkage map for Musca domestica containing 35 microsatellite plus six visible markers. We report the development of 33 new microsatellite markers of which 19 are included in the linkage map. Two hundred and thirty-six F2 individuals were genotyped from three crosses yielding a linkage map consisting of five linkage groups that represent the five autosomes of the housefly. The map covers a total of 229.6 cM with an average marker spacing of 4.4 cM spanning approximately 80.2% of the genome. We found up to 29% recombination in male houseflies in contrast to most previous studies. The linkage map will add to genetic studies of the housefly.
我们展示了第一张家蝇分子标记连锁图谱，其中包含35个微卫星标记和6个可见标记。我们报告了33个新微卫星标记的开发情况，其中19个被纳入连锁图谱。对来自三个杂交组合的236个F2个体进行了基因分型，得到了一个由五个连锁群组成的连锁图谱，代表家蝇的五条常染色体。该图谱总长229.6厘摩，平均标记间距为4.4厘摩，覆盖了约80.2%的基因组。与之前的大多数研究不同，我们发现雄性家蝇中重组率高达29%。该连锁图谱将有助于家蝇的遗传学研究。
