Kim J S, McKinnis V S, Nawrocki A, White S R
Department of Medicine, University of Chicago, Illinois 60637, USA.
Am J Respir Cell Mol Biol. 1998 Jan;18(1):66-74. doi: 10.1165/ajrcmb.18.1.2740.
Repair of the airway epithelium after injury involves cell proliferation, migration, and spreading into the injury site. The growth factor, epidermal growth factor (EGF), elicits proliferation of many epithelial cell types in vitro and in vivo, including airways epithelium. However, its effects on cell migration and spreading are less clear. We studied the effects of EGF on guinea-pig tracheal epithelial cell (GPTEC) chemotaxis and migration during wound repair. Primary GPTEC were allowed to migrate through a gelatin-coated filter for 6 h in a chemotaxis chamber, after which the number of migrated cells were counted. EGF elicited migration of GPTEC that was substantial and concentration-dependent. Treatment with EGF accelerated closure of small wounds in confluent epithelial monolayers substantially as measured by video microscopy over 24 h. These effects of EGF were concentration-dependent and seen in monolayer wounds of different size. Effects of EGF did not depend on the underlying matrix on which cells were grown; cells grown on laminin, fibronectin, or collagen had similar wound closure velocities in response to EGF. Early effects of EGF on wound closure were not due to cell proliferation at the wound edge. These data demonstrate that EGF elicits both chemotaxis and migration of airway epithelial cells in culture.
损伤后气道上皮的修复涉及细胞增殖、迁移以及向损伤部位的铺展。生长因子表皮生长因子（EGF）在体外和体内均可引发多种上皮细胞类型的增殖，包括气道上皮细胞。然而，其对细胞迁移和铺展的影响尚不清楚。我们研究了EGF在伤口修复过程中对豚鼠气管上皮细胞（GPTEC）趋化性和迁移的影响。原代GPTEC在趋化性小室中通过明胶包被的滤膜迁移6小时，之后对迁移细胞数量进行计数。EGF引发了GPTEC的迁移，这种迁移显著且呈浓度依赖性。通过视频显微镜在24小时内测量发现，用EGF处理可显著加速汇合上皮单层中小伤口的闭合。EGF的这些作用呈浓度依赖性，且在不同大小的单层伤口中均可见。EGF对伤口闭合的作用不依赖于细胞生长的基础基质；在层粘连蛋白、纤连蛋白或胶原蛋白上生长的细胞对EGF的反应具有相似的伤口闭合速度。EGF对伤口闭合的早期作用并非由于伤口边缘的细胞增殖。这些数据表明，EGF在培养中可引发气道上皮细胞的趋化性和迁移。
