Silversand C, Haux C
Department of Zoophysiology, Göteborg University, Sweden.
J Chromatogr B Biomed Sci Appl. 1997 Dec 5;703(1-2):7-14. doi: 10.1016/s0378-4347(97)00385-x.
An improved straight-phase HPLC method for the separation and quantification of lipid classes is described. Two binary gradient solvent systems were used, one for polar and one for neutral lipids, and detection was performed with a light-scattering detector. The developed HPLC methods were highly reproducible and allowed base-line separation of all investigated polar lipid classes (phosphatidic acid, diphosphatidylglycerol. phosphatidylethanolamine, phosphatidylcholine, sphingomyelin, phosphatidylserine, phosphatidylinositol and lysophosphatidylcholine) and neutral lipid classes (triacylglycerol, free fatty acid, diacylglycerol, cholesterol and monoacylglycerol) except of cholesterol ester and wax ester. Application of the chromatographic systems demonstrated that the methods are suitable for quantitative analysis of the major lipid classes present in lipid extracts from livers and eggs of Atlantic salmon (Salmo salar).
本文描述了一种改进的正相高效液相色谱法，用于脂质类别的分离和定量分析。使用了两种二元梯度溶剂系统，一种用于极性脂质，一种用于中性脂质，并采用光散射检测器进行检测。所开发的高效液相色谱方法具有高度的重现性，能够对所有研究的极性脂质类别（磷脂酸、二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、鞘磷脂、磷脂酰丝氨酸、磷脂酰肌醇和溶血磷脂酰胆碱）和中性脂质类别（三酰甘油、游离脂肪酸、二酰甘油、胆固醇和单酰甘油）进行基线分离，但胆固醇酯和蜡酯除外。色谱系统的应用表明，这些方法适用于对大西洋鲑（Salmo salar）肝脏和卵脂质提取物中主要脂质类别的定量分析。
