哺乳动物DNA聚合酶的模板特异性抑制剂。
Template specific inhibitor of mammalian DNA polymerases.
作者信息
Pitha J, Wilson S H
出版信息
Nucleic Acids Res. 1976 Mar;3(3):825-34. doi: 10.1093/nar/3.3.825.
A study of the inhibition of mouse cellular DNA polymerases by poly-nucleotides and their vinyl analogs is presented. Poly(dT)-directed poly(dA) synthesis by representatives of all three classes of cellular DNA polymerase could be completely inhibited by poly(9-vinyladenine), although higher concentrations were required in the case of the gamma class enzyme. Studies on the mechanism of the inhibition using the alpha class DNA polymerase and different templates showed that the enzyme activity was inhibited in all cases where base-pairing between the vinyl polymer and the template occurred; poly(9-vinyladenine) did not interfere with the replication of templates to which it does not bind. The inhibition occurred shortly after addition of poly(9-vinyladenine) to ongoing reactions, yet the enzyme was not displaced from the template - primer complex.
本文介绍了一项关于多核苷酸及其乙烯基类似物对小鼠细胞DNA聚合酶抑制作用的研究。聚（9-乙烯基腺嘌呤）可完全抑制所有三类细胞DNA聚合酶代表物的聚（dT）指导的聚（dA）合成，尽管对于γ类酶需要更高的浓度。使用α类DNA聚合酶和不同模板对抑制机制的研究表明，在乙烯基聚合物与模板之间发生碱基配对的所有情况下，酶活性均受到抑制；聚（9-乙烯基腺嘌呤）不会干扰其不结合的模板的复制。在向正在进行的反应中添加聚（9-乙烯基腺嘌呤）后不久就发生了抑制作用，但酶并未从模板-引物复合物中被置换出来。
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