哺乳动物DNA聚合酶的模板特异性抑制剂。

Template specific inhibitor of mammalian DNA polymerases.

作者信息

Pitha J, Wilson S H

出版信息

Nucleic Acids Res. 1976 Mar;3(3):825-34. doi: 10.1093/nar/3.3.825.

DOI:10.1093/nar/3.3.825

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342943/

Abstract

A study of the inhibition of mouse cellular DNA polymerases by poly-nucleotides and their vinyl analogs is presented. Poly(dT)-directed poly(dA) synthesis by representatives of all three classes of cellular DNA polymerase could be completely inhibited by poly(9-vinyladenine), although higher concentrations were required in the case of the gamma class enzyme. Studies on the mechanism of the inhibition using the alpha class DNA polymerase and different templates showed that the enzyme activity was inhibited in all cases where base-pairing between the vinyl polymer and the template occurred; poly(9-vinyladenine) did not interfere with the replication of templates to which it does not bind. The inhibition occurred shortly after addition of poly(9-vinyladenine) to ongoing reactions, yet the enzyme was not displaced from the template - primer complex.

摘要

本文介绍了一项关于多核苷酸及其乙烯基类似物对小鼠细胞DNA聚合酶抑制作用的研究。聚（9-乙烯基腺嘌呤）可完全抑制所有三类细胞DNA聚合酶代表物的聚（dT）指导的聚（dA）合成，尽管对于γ类酶需要更高的浓度。使用α类DNA聚合酶和不同模板对抑制机制的研究表明，在乙烯基聚合物与模板之间发生碱基配对的所有情况下，酶活性均受到抑制；聚（9-乙烯基腺嘌呤）不会干扰其不结合的模板的复制。在向正在进行的反应中添加聚（9-乙烯基腺嘌呤）后不久就发生了抑制作用，但酶并未从模板-引物复合物中被置换出来。

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引用本文的文献

1

Template specific inhibitors of E. coli RNA polymerase.大肠杆菌RNA聚合酶的模板特异性抑制剂。

Nucleic Acids Res. 1978 Mar;5(3):691-6. doi: 10.1093/nar/5.3.691.

本文引用的文献

1

Deoxyadenylate-rich sequences in mammalian DNA.哺乳动物DNA中富含脱氧腺苷酸的序列。

FEBS Lett. 1972 May 1;22(2):157-160. doi: 10.1016/0014-5793(72)80033-4.

2

Vinyl analogs of polynucleotides.多核苷酸的乙烯基类似物。

Biochemistry. 1971 Dec 7;10(25):4595-602. doi: 10.1021/bi00801a001.

3

Polynucleotide analogue inhibition of messenger-stimulated transfer RNA binding to ribosomes.多核苷酸类似物对信使刺激的转运RNA与核糖体结合的抑制作用。

Biochim Biophys Acta. 1971 Dec 30;254(3):452-6. doi: 10.1016/0005-2787(71)90879-3.

4

Polynucleotide sequences in eukaryotic DNA and RNA that form ribonuclease-resistant complexes with polyuridylic acid.真核生物DNA和RNA中的多核苷酸序列，其与聚尿苷酸形成抗核糖核酸酶的复合物。

J Mol Biol. 1974 May 5;85(1):75-86. doi: 10.1016/0022-2836(74)90130-2.

5

Deoxyadenylate-rich and deoxyguanylate-rich regions in mammalian DNA.哺乳动物DNA中富含脱氧腺苷酸和富含脱氧鸟苷酸的区域。

J Mol Biol. 1974 May 5;85(1):19-39. doi: 10.1016/0022-2836(74)90126-0.

6

Hydrolysis of template and newly synthesized deoxyribonucleic acid by the 3' to 5' exonuclease activity of the T4 deoxyribonucleic acid polymerase.T4 脱氧核糖核酸聚合酶的 3' 至 5' 核酸外切酶活性对模板及新合成的脱氧核糖核酸的水解作用。

J Biol Chem. 1972 Jun 10;247(11):3393-404.

7

Analysis of long pyrimidine polynucleotides in HeLa cell nuclear DNA: absence of polydeoxythymidylate.对HeLa细胞核DNA中长嘧啶多核苷酸的分析：无聚脱氧胸苷酸。

Proc Natl Acad Sci U S A. 1973 Aug;70(8):2189-92. doi: 10.1073/pnas.70.8.2189.

8

Structures for the polynucleotide complexes poly(dA) with poly (dT) and poly(dT) with poly(dA) with poly (dT).聚（dA）与聚（dT）以及聚（dT）与聚（dA）和聚（dT）形成的多核苷酸复合物的结构

J Mol Biol. 1974 Sep 15;88(2):509-21. doi: 10.1016/0022-2836(74)90498-7.

9

Nuclear deoxyribonucleic acid polymerase. Purification and properties of the homogeneous enzyme from human KB cells.核脱氧核糖核酸聚合酶。从人KB细胞中纯化及鉴定该纯酶的性质。

J Biol Chem. 1974 Feb 10;249(3):841-50.

10

Poly U tracts absent from viral RNA.病毒RNA中不存在多聚U序列。

Nat New Biol. 1972 Nov 8;240(97):43-5. doi: 10.1038/newbio240043a0.

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