受张力作用下短杆菌肽通道的动力学
Gramicidin channel kinetics under tension.
作者信息
Goulian M, Mesquita O N, Fygenson D K, Nielsen C, Andersen O S, Libchaber A
机构信息
Center for Studies in Physics and Biology, Rockefeller University, New York, New York 10021, USA.
出版信息
Biophys J. 1998 Jan;74(1):328-37. doi: 10.1016/S0006-3495(98)77790-2.
Abstract
We have measured the effect of tension on dimerization kinetics of the channel-forming peptide gramicidin A. By aspirating large unilamellar vesicles into a micropipette electrode, we are able to simultaneously monitor membrane tension and electrical activity. We find that the dimer formation rate increases by a factor of 5 as tension ranges from 0 to 4 dyn/cm. The dimer lifetime also increases with tension. This behavior is well described by a phenomenological model of membrane elasticity in which tension modulates the mismatch in thickness between the gramicidin dimer and membrane.
摘要
我们测量了张力对形成通道的肽短杆菌肽A二聚化动力学的影响。通过将大单层囊泡吸入微量移液器电极，我们能够同时监测膜张力和电活性。我们发现，当张力从0达因/厘米变化到4达因/厘米时，二聚体形成速率增加了5倍。二聚体寿命也随张力增加。这种行为可以用膜弹性的唯象模型很好地描述，其中张力调节短杆菌肽二聚体与膜之间厚度的不匹配。
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