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本文引用的文献

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Gas-Phase Protonation Thermodynamics of Biological Lipids: Experiment, Theory, and Implications.生物脂质的气相质子化热力学：实验、理论与意义。
Anal Chem. 2020 Aug 4;92(15):10365-10374. doi: 10.1021/acs.analchem.0c00613. Epub 2020 Jul 19.
2
Revealing the Specificity of a Range of Antimicrobial Peptides in Lipid Nanodiscs by Native Mass Spectrometry.通过天然质谱揭示一系列抗菌肽在脂质纳米盘中的特异性。
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Native Mass Spectrometry of Antimicrobial Peptides in Lipid Nanodiscs Elucidates Complex Assembly.脂质纳米盘中抗菌肽的天然质谱阐明了复杂的组装。
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Relative interfacial cleavage energetics of protein complexes revealed by surface collisions.通过表面碰撞揭示蛋白质复合物的相对界面断裂能。
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Surface-Induced Dissociation of Noncovalent Protein Complexes in an Extended Mass Range Orbitrap Mass Spectrometer.在扩展质量范围轨道阱质谱仪中表面诱导的非共价蛋白质复合物的解离。
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Apolipoprotein A1 Forms 5/5 and 5/4 Antiparallel Dimers in Human High-density Lipoprotein.载脂蛋白 A1 在人高密度脂蛋白中形成 5/5 和 5/4 反平行二聚体。
Mol Cell Proteomics. 2019 May;18(5):854-864. doi: 10.1074/mcp.RA118.000878. Epub 2019 Jan 18.
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MetaUniDec: High-Throughput Deconvolution of Native Mass Spectra.MetaUniDec：高通量天然质谱的去卷积。
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The application of ion-mobility mass spectrometry for structure/function investigation of protein complexes.离子淌度质谱在蛋白质复合物结构/功能研究中的应用。
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65 ASMS Conference on Mass Spectrometry and Allied Topics.第65届质谱及相关主题会议
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利用表面诱导解离质谱法探测纳米盘的结构。

Probing the structure of nanodiscs using surface-induced dissociation mass spectrometry.

机构信息

Department of Chemistry and Biochemistry and Resource for Native Mass Spectrometry Guided Structural Biology, The Ohio State University, Columbus, OH, USA.

出版信息

Chem Commun (Camb). 2020 Dec 21;56(100):15651-15654. doi: 10.1039/d0cc05531j. Epub 2020 Dec 8.

DOI:10.1039/d0cc05531j
PMID:33355562
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7943047/
Abstract

In the study of membrane proteins and antimicrobial peptides, nanodiscs have emerged as a valuable membrane mimetic to solubilze these molecules in a lipid bilayer. We present the structural characterization of nanodiscs using native mass spectrometry and surface-induced dissociation, which are powerful tools in structural biology.

摘要

在膜蛋白和抗菌肽的研究中，纳米盘作为一种有价值的膜模拟物，可以将这些分子溶解在脂质双层中。我们使用天然质谱法和表面诱导离解对纳米盘进行结构表征，这是结构生物学中的有力工具。