Michurina T V, Khrushchov N G
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow.
Int J Dev Biol. 1997 Dec;41(6):817-33.
The principal approach was to study hemopoiesis on different stromal cell underlayers (fibroblasts or fibroblast-like cells covering a foreign body implanted into the peritoneal cavity of mice or other rodents) after intraperitoneal transplantation of syngeneic, allogeneic, and xenogeneic hemopoietic cells. The data obtained are compared with the results of experiments on repopulation of ectopic hemopoietic territories (under the mouse kidney capsule) by syngeneic and xenogeneic hemopoietic cells. Competitive cell interactions are described that occur during repopulation of the hemopoietic stroma or formation of the hemopoietic foci on cellulose acetate membranes (CAMs) in the peritoneal cavity of irradiated mice by genetically different hemopoietic cells transplanted to these animals (multicomponent radiation chimeras). The model of xenogeneic and multicomponent radiation chimeras was reproduced in long-term bone marrow cultures, where hemopoietic cells of different genotypes coexisted, without any competitive cell elimination. The second part of this review deals with hemopoiesis on stromal cell underlayers, formed by cells of different origin, different stages of development, and obtained from other sources. These underlayers were formed on CAMs in vitro and then transferred into the peritoneal cavity of irradiated mice, which subsequently received intraperitoneal injections of donor hemopoietic cells. Specific features of hemopoiesis on stromal underlayers formed by the following cell types are described: (1) fibroblasts from mouse embryos at different developmental stages; (2) fibroblasts from the skin, liver, and bone marrow of 17-day mouse fetuses and newborn mice; (3) fibroblasts from the monolayer cultures of mouse and rat bone marrow; (4) 3T3 cell line; (5) hepatocytes of 17-day mouse fetuses or sexually mature rats; (6) newborn mouse kidney cells; and (7) cells transgenic for the erythropoietin gene. The phenomena observed in these experiments and their probable mechanisms are discussed.
主要方法是在同基因、异基因和异种造血细胞腹腔内移植后，研究不同基质细胞底层（覆盖植入小鼠或其他啮齿动物腹腔内异物的成纤维细胞或成纤维样细胞）上的造血作用。将获得的数据与同基因和异种造血细胞对异位造血区域（小鼠肾包膜下）进行再填充的实验结果进行比较。描述了竞争性细胞相互作用，这种相互作用发生在将基因不同的造血细胞移植到受辐照小鼠腹腔内的醋酸纤维素膜（CAMs）上的造血基质再填充或造血灶形成过程中（多组分辐射嵌合体）。在长期骨髓培养中重现了异种和多组分辐射嵌合体模型，不同基因型的造血细胞在其中共存，没有任何竞争性细胞清除现象。本综述的第二部分涉及由不同来源、不同发育阶段且从其他来源获得的细胞形成的基质细胞底层上的造血作用。这些底层在体外的CAMs上形成，然后转移到受辐照小鼠的腹腔内，随后给这些小鼠腹腔注射供体造血细胞。描述了由以下细胞类型形成的基质底层上造血作用的具体特征：（1）不同发育阶段小鼠胚胎的成纤维细胞；（2）17天龄小鼠胎儿和新生小鼠皮肤、肝脏和骨髓的成纤维细胞；（3）小鼠和大鼠骨髓单层培养的成纤维细胞；（4）3T3细胞系；（5）17天龄小鼠胎儿或性成熟大鼠的肝细胞；（6）新生小鼠肾细胞；（7）促红细胞生成素基因转基因细胞。讨论了这些实验中观察到的现象及其可能的机制。
