Weijtens M J, Urlings H A, Van der Plas J
Department of the Science of Food of Animal Origin, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Lett Appl Microbiol. 2000 Jun;30(6):479-84. doi: 10.1046/j.1472-765x.2000.00750.x.
Fattening pigs are often infected with campylobacter. To eliminate campylobacter from the pig population, a top-down approach, involving the breeding and reproduction farms, seems appropriate. In order to investigate the effectiveness of a top-down approach, sows' faeces from the following farms were analysed for the presence of campylobacter: one specific pathogen free (SPF) farm, three top-breeding farms with no connection with SPF breeding, and a breeding farm repopulated with SPF sows after a period of vacancy (farm 5). The faeces samples from the SPF farm were free from campylobacter. The three top-breeding farms provided faeces samples which were 98% positive for campylobacter. However, only 22% of the faeces samples from farm 5 were positive for campylobacter. In a period of 20 months, the percentage of sows infected with campylobacter on farm 5 did not significantly increase. Genetic typing with ERIC-PCR and RFLP of campylobacter isolates from one of the top-breeding farms and from farm 5 showed a high diversity of campylobacter types. The results suggest that a campylobacter-free pig population can be established in breeding farms by combining a top-down approach (campylobacter-free top-breeding farms) with a strict regime of hygiene management.
育肥猪常感染弯曲杆菌。为了在猪群中消除弯曲杆菌，一种自上而下的方法，包括育种场和繁殖场，似乎是合适的。为了研究自上而下方法的有效性，对以下猪场母猪的粪便进行了弯曲杆菌检测：一个特定无特定病原体（SPF）猪场、三个与SPF育种无关联的顶级育种场，以及一个在空置一段时间后重新引入SPF母猪的繁殖场（猪场5）。SPF猪场的粪便样本未检测出弯曲杆菌。三个顶级育种场提供的粪便样本弯曲杆菌阳性率为98%。然而，猪场5的粪便样本中只有22%弯曲杆菌呈阳性。在20个月的时间里，猪场5感染弯曲杆菌的母猪比例没有显著增加。对其中一个顶级育种场和猪场5的弯曲杆菌分离株进行ERIC-PCR和RFLP基因分型，结果显示弯曲杆菌类型具有高度多样性。结果表明，通过将自上而下的方法（无弯曲杆菌的顶级育种场）与严格的卫生管理制度相结合，可以在育种场建立无弯曲杆菌的猪群。
