Santos A, Ortiz de Solórzano C, Vaquero J J, Peña J M, Malpica N, del Pozo F
Grupo de Bioingeniería y Telemedicina, E.T.S.I. Telecomunicación, Ciudad Universitaria, Madrid, Spain.
J Microsc. 1997 Dec;188(Pt 3):264-72. doi: 10.1046/j.1365-2818.1997.2630819.x.
This work describes a systematic evaluation of several autofocus functions used for analytical fluorescent image cytometry studies of counterstained nuclei. Focusing is the first step in the automatic fluorescence in situ hybridization analysis of cells. Thirteen functions have been evaluated using qualitative and quantitative procedures. For the last of these procedures a figure-of-merit (FOM) is defined and proposed. This new FOM takes into account five important features of the focusing function. Our results show that functions based on correlation measures have the best performance for this type of image.
这项工作描述了对几种用于经复染细胞核的分析荧光图像细胞术研究的自动聚焦功能的系统评估。聚焦是细胞自动荧光原位杂交分析的第一步。已使用定性和定量程序对13种功能进行了评估。对于这些程序中的最后一个，定义并提出了一个品质因数（FOM）。这个新的FOM考虑了聚焦功能的五个重要特征。我们的结果表明，基于相关性度量的功能对于这类图像具有最佳性能。
