Schoenfield M, Sadava D
Arch Int Pharmacodyn Ther. 1976 Feb;219(2):347-50.
Adult male rats were addicted to methadone via small dosages in their drinking water. The N-demethylation activity of microsomal mixed function oxidase in liver was determined for partially addicted (25 days of drug consumption) and fully addicted (38 days of drug consumption) rats. Compared to controls which did not receive the drug, enzyme activity expressed on a microsmal protein basis was increased in partially addicted, but not increased in fully addicted animals. Enzyme activity expressed on a liver weight basis was decreased in partially addicted animals but unchanged from contro levels in fully addicted animals. Brief (three-day) exposures to phenobarbital or high dosages of methadone led to an increase in N-demethylation activity.
成年雄性大鼠通过在饮用水中添加小剂量美沙酮使其成瘾。测定了部分成瘾（摄入药物25天）和完全成瘾（摄入药物38天）大鼠肝脏微粒体混合功能氧化酶的N-脱甲基化活性。与未接受药物的对照组相比，以微粒体蛋白为基础表示的酶活性在部分成瘾的大鼠中增加，但在完全成瘾的动物中未增加。以肝脏重量为基础表示的酶活性在部分成瘾的动物中降低，但在完全成瘾的动物中与对照水平相比没有变化。短期（三天）接触苯巴比妥或高剂量美沙酮会导致N-脱甲基化活性增加。
