Riley H D, Macnab J, Farrell T J, Cohn K
Research & Development Service, VA Medical & Regional Office Center, White River Junction, VT 05009, USA.
Carcinogenesis. 1997 Dec;18(12):2453-5. doi: 10.1093/carcin/18.12.2453.
We have performed differential display comparing gene expression from cell lines derived from human colorectal tumors. The cell lines were selected for study based on their ability to form metastases following injection into athymic mice. One gene which was expressed exclusively by the metastatic lines was identified as human acylphosphatase (e.c. 3.6.1.7, acylphosphate phosphohydrolase). The expression of this gene was confirmed by RT-PCR using gene-specific primers. This gene product catalyzes the hydrolysis of phosphorylated intermediates of Na+/K(+)-ATPase and of Ca(2+)-ATPases of mammalian cells. Changes in the activity of the Na+/K(+)-ATPase pump, regulated by acylphosphatase, have been previously reported in chemically-induced colonic tumors. The differential expression of this gene in the human metastatic colorectal lines suggests it may be involved in the metastatic phenotype.
我们进行了差异显示，比较了源自人类结肠直肠肿瘤的细胞系的基因表达。这些细胞系是根据其注入无胸腺小鼠后形成转移灶的能力来选择进行研究的。一个仅由转移细胞系表达的基因被鉴定为人酰基磷酸酶（酶编号3.6.1.7，酰基磷酸磷酸水解酶）。使用基因特异性引物通过RT-PCR证实了该基因的表达。该基因产物催化哺乳动物细胞中Na+/K(+)-ATP酶和Ca(2+)-ATP酶的磷酸化中间体的水解。先前已报道在化学诱导的结肠肿瘤中，由酰基磷酸酶调节的Na+/K(+)-ATP酶泵活性发生了变化。该基因在人类转移性结肠直肠细胞系中的差异表达表明它可能与转移表型有关。
