Kanda Y, Chiba S, Suzuki T, Kami M, Yazaki Y, Hirai H
Department of Cell Therapy and Transplantation Medicine, Faculty of Medicine, University of Tokyo, Japan.
Br J Haematol. 1998 Jan;100(1):222-5. doi: 10.1046/j.1365-2141.1998.00518.x.
Cytomegalovirus (CMV) disease is a serious complication after bone marrow transplantation (BMT). Recently, semi-quantitative polymerase chain reaction (SQ-PCR) analysis for CMV-DNA has been developed. We compared the time course of SQ-PCR and that of antigenaemia assay after BMT. Our findings suggested that SQ-PCR is more sensitive than antigenaemia assay in the detection of CMV infection and that quantitation of CMV-DNA could discriminate patients with positive PCR but without the risk for CMV disease. We considered that SQ-PCR could be a useful guide for starting pre-emptive therapy against CMV disease.
巨细胞病毒（CMV）疾病是骨髓移植（BMT）后的一种严重并发症。最近，已开发出用于CMV-DNA的半定量聚合酶链反应（SQ-PCR）分析。我们比较了骨髓移植后SQ-PCR和抗原血症检测的时间进程。我们的研究结果表明，在检测CMV感染方面，SQ-PCR比抗原血症检测更敏感，并且CMV-DNA定量可以区分PCR阳性但无CMV疾病风险的患者。我们认为SQ-PCR可为启动针对CMV疾病的抢先治疗提供有用的指导。
