Gallo C, Orlassino R, Demarchi A, Vallero P, Vineis C
U.O.A. di Anatomia Patologica ed Istocitopatologia, Ospedale Civile di Ivrea, Piazza della Credenza 2, I-10015 Ivrea, TO, Italia.
Pathologica. 2002 Aug;94(4):176-80. doi: 10.1007/s102420200028.
The aim of this study was to compare the grade of concordance between three methods to evaluate and to quantify the expression of estrogen and progesterone receptors in 180 cases of breast cancer. The methods compared were dextran-coated charcoal (DCC) method, immunochemical analysis (ICA), and immunohistochemistry (IHC). The grades of concordance are so summarized: (1) 77.8% for estrogen receptors (ER) and progesterone receptors (PgR) with DCC and ICA methods (r = 0.53 and r = 0.64, respectively); (2) 83.3% for ER (r = 0.53) and 75.6% for PgR (r = 0.64) with DCC and IHC methods; (3) 87.8% for ER (r = 0.98) and 86.7% for PgR (r = 0.99) with ICA and IHC methods. Nowadays, the technique carried out on tissue sections provides the most reliable information. The IHC method performed on formalin-fixed and paraffin-embedded tissue is preferred in laboratories with a high work load because it is easy and inexpensive and can be automated.
本研究的目的是比较三种评估和量化180例乳腺癌中雌激素和孕激素受体表达的方法之间的一致性程度。所比较的方法为葡聚糖包被活性炭（DCC）法、免疫化学分析（ICA）和免疫组织化学（IHC）。一致性程度总结如下：（1）DCC法与ICA法检测雌激素受体（ER）和孕激素受体（PgR）的一致性为77.8%（r分别为0.53和0.64）；（2）DCC法与IHC法检测ER的一致性为83.3%（r = 0.53），检测PgR的一致性为75.6%（r = 0.64）；（3）ICA法与IHC法检测ER的一致性为87.8%（r = 0.98），检测PgR的一致性为86.7%（r = 0.99）。目前，在组织切片上进行的技术可提供最可靠的信息。在工作量大的实验室中，对福尔马林固定石蜡包埋组织进行的IHC法是首选，因为它简便、成本低且可实现自动化。
