Ghanim M, Morin S, Zeidan M, Czosnek H
Department of Field Crops and Genetics, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot, Israel.
Virology. 1998 Jan 20;240(2):295-303. doi: 10.1006/viro.1997.8937.
The whitefly Bemisia tabaci is the only vector of the tomato yellow leaf curl geminivirus (TYLCV). The insect transmits the virus in a persistent-circulative manner. TYLCV DNA was detected by polymerase chain reaction and by Southern blot hybridization in progeny (eggs, first and second instars, adults) of single viruliferous whiteflies that developed on eggplant or on cotton (two TYLCV nonhost plants). Furthermore, TYLCV DNA was present in the progeny of insects that had acquired the virus through the egg. The adult progeny of the viruliferous insects and their own progeny were able to infect tomato test plants, producing typical disease symptoms. Ovaries and maturing eggs of viruliferous insects contained viral DNA, as did eggs laid by viruliferous insects maintained on an artificial diet Eggs laid by nonviruliferous whiteflies on cotton plants previously caged with viruliferous insects did not acquire viral DNA from the plant. Hence, TYLCV can be transmitted through the egg for at least two generations. In the absence of an available plant host, the whitefly may serve as a reservoir of the virus between growing seasons.
烟粉虱是番茄黄化曲叶双生病毒（TYLCV）的唯一传播媒介。这种昆虫以持久性循环方式传播病毒。通过聚合酶链反应和Southern印迹杂交，在取食茄子或棉花（两种TYLCV非寄主植物）的单个带毒烟粉虱的后代（卵、一龄和二龄若虫、成虫）中检测到了TYLCV DNA。此外，通过卵获得病毒的昆虫后代中也存在TYLCV DNA。带毒昆虫的成虫后代及其自身后代能够感染番茄试验植株，产生典型的病害症状。带毒昆虫的卵巢和成熟卵中含有病毒DNA，以人工饲料饲养的带毒昆虫所产的卵中也含有病毒DNA。在先前用带毒昆虫笼罩的棉花植株上，无毒烟粉虱所产的卵并未从植株上获得病毒DNA。因此，TYLCV可通过卵传播至少两代。在没有可用植物寄主的情况下，烟粉虱可能在生长季节之间充当病毒的储存宿主。
