Mayo K, Pretolani S, Gasbarrini G, Ghironzi G, Megraud F
Laboratoire de Bactériologie-Enfants, Hôpital Pellegrin, Bordeaux, France.
Clin Diagn Lab Immunol. 1998 Jan;5(1):70-3. doi: 10.1128/CDLI.5.1.70-73.1998.
The heterogeneity of the immune response to Helicobacter pylori has always been noticed but has never been evaluated by obtaining a quantitative measure. For this purpose, sera were tested by enzyme-linked immunosorbent assay, and 207 positive serum specimens were subsequently tested by immunoblotting. The presence or absence of six specific bands was noted. The homology of the different profiles of bands was measured by calculating the Dice coefficient, and a dendrogram was constructed. Thirty-four profiles were found, with each profile containing from 1 to 43 serum specimens. At a level of 72% similarity, 115 of the serum specimens studied fell into eight profiles. At a level of 48% similarity, 186 of the serum specimens studied fell into 22 profiles. The difference in immunoblot profiles was probably linked to the host immune response, but infection with strains carrying different antigens cannot be ruled out.
对幽门螺杆菌免疫反应的异质性一直受到关注，但从未通过获得定量测量来进行评估。为此，采用酶联免疫吸附测定法检测血清，随后对207份阳性血清标本进行免疫印迹检测。记录六条特异性条带的有无。通过计算迪西系数来测量不同条带图谱的同源性，并构建树状图。共发现34种图谱，每种图谱包含1至43份血清标本。在相似度为72%的水平上，所研究的115份血清标本归入8种图谱。在相似度为48%的水平上，所研究的186份血清标本归入22种图谱。免疫印迹图谱的差异可能与宿主免疫反应有关，但不能排除感染携带不同抗原的菌株。
