Magnarelli L A, Ijdo J W, Padula S J, Flavell R A, Fikrig E
Department of Entomology, The Connecticut Agricultural Experiment Station, New Haven, Connecticut 06504, USA.
J Clin Microbiol. 2000 May;38(5):1735-9. doi: 10.1128/JCM.38.5.1735-1739.2000.
Class-specific enzyme-linked immunosorbent assays (ELISAs) with purified recombinant antigens of Borrelia burgdorferi sensu stricto and Western blot analyses with whole cells of this spirochete were used to test human sera to determine which antigens were diagnostically important. In analyses for immunoglobulin M (IgM) antibodies, 14 (82%) of 17 serum samples from persons who had erythema migrans reacted positively by an ELISA with one or more recombinant antigens. There was frequent antibody reactivity to protein 41-G (p41-G), outer surface protein C (OspC), and OspF antigens. In an ELISA for IgG antibodies, 13 (87%) of 15 serum samples had antibodies to recombinant antigens; reactivity to p22, p39, p41-G, OspC, and OspF antigens was frequent. By both ELISAs, serum specimens positive for OspB, OspE, and p37 were uncommon. Analyses of sera obtained from persons who were suspected of having human granulocytic ehrlichiosis (HGE) but who lacked antibodies to ehrlichiae revealed IgM antibodies to all recombinant antigens of B. burgdorferi except OspB and IgG antibodies to all antigens except OspE. Immunoblotting of sera from the study group of individuals suspected of having HGE reaffirmed antibody reactivity to multiple antigens of B. burgdorferi. There was minor cross-reactivity when sera from healthy subjects or persons who had syphilis, oral infections, or rheumatoid arthritis were tested by ELISAs with p37, p41-G, OspB, OspC, OspE, and OspF antigens. Although the results of class-specific ELISAs with recombinant antigens were comparable to those recorded for assays with whole-cell antigen and for individuals with confirmed clinical diagnoses of Lyme borreliosis, immunoblotting is still advised as an adjunct procedure, particularly when there are low antibody titers by an ELISA.
采用针对狭义伯氏疏螺旋体纯化重组抗原的特异性类别酶联免疫吸附测定（ELISA）以及用该螺旋体全细胞进行的蛋白质印迹分析来检测人血清，以确定哪些抗原具有诊断重要性。在免疫球蛋白M（IgM）抗体分析中，17例患有游走性红斑患者的血清样本中有14例（82%）通过ELISA与一种或多种重组抗原呈阳性反应。对蛋白质41-G（p41-G）、外膜蛋白C（OspC）和OspF抗原常有抗体反应。在针对IgG抗体的ELISA中，15例血清样本中有13例（87%）对重组抗原有抗体；对p22、p39、p41-G、OspC和OspF抗原常有反应。通过两种ELISA方法，OspB、OspE和p37血清标本呈阳性的情况并不常见。对疑似患有人类粒细胞埃立克体病（HGE）但缺乏抗埃立克体抗体的人所采集的血清分析显示，除OspB外，对伯氏疏螺旋体所有重组抗原均有IgM抗体，除OspE外，对所有抗原均有IgG抗体。对疑似患有HGE的研究组个体血清进行的免疫印迹分析再次证实了对伯氏疏螺旋体多种抗原的抗体反应。当用p37、p41-G、OspB、OspC、OspE和OspF抗原通过ELISA检测健康受试者或患有梅毒、口腔感染或类风湿关节炎患者的血清时，存在轻微交叉反应。尽管采用重组抗原的特异性类别ELISA结果与用全细胞抗原检测以及临床确诊莱姆病患者的检测结果相当，但仍建议将免疫印迹作为辅助程序，特别是当ELISA抗体滴度较低时。
