Suzuki Y, Nishimura Y, Tanihara M, Suzuki K, Nakamura T, Shimizu Y, Yamawaki Y, Kakimaru Y
Department of Plastic and Reconstructive Surgery, Faculty of Medicine, Kyoto University, Japan.
J Biomed Mater Res. 1998 Feb;39(2):317-22. doi: 10.1002/(sici)1097-4636(199802)39:2<317::aid-jbm20>3.0.co;2-8.
Calcium alginate dressings have beneficial effects on wound healing by providing a moist wound environment. However, cytotoxicity and the nonbiodegradable nature of calcium alginate dressings induce unresolved chronic foreign-body reaction. In this study, a novel freeze-dried alginate gel dressing (AGA-100) low in calcium ions was evaluated for cytotoxicity to L929 cells in vitro and in full-thickness pig wounds in vivo. Cytotoxicity testing on L929 cells showed the cytocompatibility of AGA-100 extracts, while extracts from Kaltostat, a well-established alginate dressing, induced cytopathic effects. In an in vivo study using pigskin, AGA-100, Kaltostat, and gauze were applied on 1-in-diameter circular full-thickness wounds on the back of pigs and the time course of wound closure was evaluated. Kaltostat and gauze dressings were used as controls. For histologic evaluation, wound tissue was harvested on day 18. AGA-100-treated wounds showed rapid wound closure compared to control wounds on day 15. Foreign-body reaction was marked in Kaltostat- and gauze-treated wounds, and differed significantly from AGA-100-treated wounds. Based on these data, AGA-100 could reduce the cytotoxicity to fibroblasts and foreign-body reaction that have been observed with currently available calcium alginate dressings; it was also found to be useful as an alginate dressing.
藻酸钙敷料通过提供湿润的伤口环境对伤口愈合具有有益作用。然而，藻酸钙敷料的细胞毒性和不可生物降解的性质会引发未解决的慢性异物反应。在本研究中，对一种新型的低钙离子冻干藻酸盐凝胶敷料（AGA - 100）进行了体外对L929细胞的细胞毒性以及体内在猪全层伤口中的评估。对L929细胞的细胞毒性测试显示了AGA - 100提取物的细胞相容性，而一种成熟的藻酸盐敷料Kaltostat的提取物则诱导了细胞病变效应。在一项使用猪皮的体内研究中，将AGA - 100、Kaltostat和纱布应用于猪背部直径为1英寸的圆形全层伤口上，并评估伤口闭合的时间进程。使用Kaltostat和纱布敷料作为对照。为了进行组织学评估，在第18天采集伤口组织。与第15天的对照伤口相比，用AGA - 100处理的伤口显示出更快的伤口闭合。在Kaltostat和纱布处理的伤口中出现明显的异物反应，且与AGA - 100处理的伤口有显著差异。基于这些数据，AGA - 100可以降低对成纤维细胞的细胞毒性以及目前可用藻酸钙敷料所观察到的异物反应；还发现它作为一种藻酸盐敷料很有用。
