Nizioł Martyna, Paleczny Justyna, Junka Adam, Shavandi Amin, Dawiec-Liśniewska Anna, Podstawczyk Daria
Department of Process Engineering and Technology of Polymer and Carbon Materials, Faculty of Chemistry, Wroclaw University of Science and Technology, Norwida 4/6, 50-373 Wroclaw, Poland.
Department of Pharmaceutical Microbiology and Parasitology, Wroclaw Medical University, 50-556 Wroclaw, Poland.
Bioengineering (Basel). 2021 Jun 8;8(6):79. doi: 10.3390/bioengineering8060079.
Thermoresponsive hydrogel-based wound dressings with an incorporated antimicrobial agent can be fabricated employing 3D printing technology. A novel printable ink containing poly(N-isopropylacrylamide) (PNIPAAm) precursors, sodium alginate (ALG), methylcellulose (MC) that is laden with a mixture of octenidine dihydrochloride and 2-phenoxyethanol (Octenisept, OCT) possess accurate printability and shape fidelity. This study also provides the protocol of ink's use for the 3D printing of hydrogel scaffolds. The hydrogel's physicochemical properties and drug release profiles from the hydrogel specimens to the external solution have been determined at two temperatures (20 and 37 °C). The release test showed a sustained OCT delivery into ultrapure water and the PBS solution. The temperature-responsive hydrogel exhibited antimicrobial activity against , , and and demonstrated non-cytotoxicity towards fibroblasts. The thermoresponsive behavior along with biocompatibility, antimicrobial activity, and controlled drug release make this hydrogel a promising class of materials for wound dressing applications.
采用3D打印技术可以制造出含有抗菌剂的基于热响应水凝胶的伤口敷料。一种新型可打印墨水，包含聚（N-异丙基丙烯酰胺）（PNIPAAm）前体、海藻酸钠（ALG）、负载有二盐酸奥替尼啶和2-苯氧基乙醇混合物（Octenisept，OCT）的甲基纤维素（MC），具有精确的可打印性和形状保真度。本研究还提供了该墨水用于水凝胶支架3D打印的方案。已在两个温度（20和37°C）下测定了水凝胶的物理化学性质以及水凝胶样品向外部溶液的药物释放曲线。释放试验表明奥替尼啶持续释放到超纯水和PBS溶液中。该温度响应性水凝胶对金黄色葡萄球菌、大肠杆菌和白色念珠菌具有抗菌活性，并对成纤维细胞表现出无细胞毒性。热响应行为以及生物相容性、抗菌活性和可控药物释放使这种水凝胶成为伤口敷料应用中一类有前景的材料。
