Carrell D T, Kuneck P H, Peterson C M, Hatasaka H H, Jones K P, Campbell B F
Division of Urology, University of Utah School of Medicine, Salt Lake City 84132, USA.
Fertil Steril. 1998 Jan;69(1):122-6. doi: 10.1016/s0015-0282(97)00446-9.
To evaluate pregnancy rates (PRs) in women undergoing artificial insemination with sperm alternately prepared by one of five techniques: sperm washing, Percoll gradient centrifugation, swim-up, swim-down, or refrigeration/heparin treatment.
Each treatment group alternated in a different order through the five sperm preparations. Pregnancy rates were compared for each sperm preparation.
Two infertility centers, one located in an academic institution and the other a regional hospital.
PATIENT(S): Three hundred sixty-three women undergoing 898 artificial inseminations with husband semen with a progressive motile sperm count of >20 million sperm per mL were randomly placed in the five treatment groups.
MAIN OUTCOME MEASURE(S): Pregnancy rates.
RESULT(S): The overall ongoing PR per insemination was 9.7% (87/898), including 6.12% for natural cycles (n = 196), 12.8% for clomiphene citrate-stimulated cycles (n = 101), and 10.3% for gonadotropin-stimulated cycles (n = 601). The highest ongoing PRs for sperm preparations followed the swim-up technique (13.2%, 26/197) and the Percoll gradient centrifugation technique (12.7%, 26/204).
CONCLUSION(S): These data suggest that the swim-up and Percoll gradient preparations result in higher PRs than the wash, swim-down, and refrigeration/heparin techniques.
评估采用五种技术之一交替制备精子后接受人工授精的女性的妊娠率（PRs），这五种技术分别为：精子洗涤、Percoll梯度离心、上游法、下游法或冷藏/肝素处理。
每个治疗组以不同顺序交替进行这五种精子制备方法。比较每种精子制备方法的妊娠率。
两个不孕不育中心，一个位于学术机构，另一个是地区医院。
363名接受898次丈夫精液人工授精的女性，其精液中进行性运动精子计数>2000万/mL，被随机分为五个治疗组。
妊娠率。
每次授精的总体持续妊娠率为9.7%（87/898），其中自然周期为6.12%（n = 196），枸橼酸氯米芬刺激周期为12.8%（n = 101），促性腺激素刺激周期为10.3%（n = 601）。精子制备方法中持续妊娠率最高的是上游法（13.2%，26/197）和Percoll梯度离心法（12.7%，26/204）。
这些数据表明，与洗涤、下游法和冷藏/肝素技术相比，上游法和Percoll梯度制备法能带来更高的妊娠率。
