Barbiers A R, Neff A W
J Assoc Off Anal Chem. 1976 Jul;59(4):849-54.
Methods for screening and for confirming residues of lincomycin in animal tissues, both sensitive to 0.1 ppm, are described. In the screening technique, residues are extracted, cleaned up by solvent partition, and detected by microbiological plate assay. In the confirmatory technique, residues are cleaned up on an unfunctionalized macroreticular-type resin column, concentrated, chromatographed on thin layer plates, and detected by bioautography. This system was effective in identifying lincomycin in the presence of 20 other antibiotics and chemicals used in the animal health industry.
本文描述了对动物组织中林可霉素残留进行筛查和确证的方法，这两种方法对0.1 ppm的残留均敏感。在筛查技术中，残留被提取出来，通过溶剂分配进行净化，然后用微生物平板法进行检测。在确证技术中，残留先在未官能化的大孔网状型树脂柱上进行净化，浓缩后在薄层板上进行色谱分析，最后通过生物自显影法进行检测。该系统在存在动物保健行业使用的其他20种抗生素和化学品的情况下，能够有效地鉴定出林可霉素。
