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使用亚硒酸盐增菌肉汤从粪便中检测沙门氏菌:省级公共卫生实验室一年经验报告

Use of selenite enrichment broth for the detection of Salmonella from stool: a report of one year experience at a provincial public health laboratory.

作者信息

Forward K R, Rainnie B J

机构信息

Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Centre, Halifax, Nova Scotia, Canada.

出版信息

Diagn Microbiol Infect Dis. 1997 Dec;29(4):215-7. doi: 10.1016/s0732-8893(97)00163-6.

Abstract

Several references recommend that selenite enrichment be used only in stool cultures from suspect carriers, during outbreaks, and in other special circumstances. To determine the impact of such an approach, we examined results from 3977 specimens cultured by our laboratory. Epidemiological information was collected from physicians and the public health department. Salmonella spp. were identified in 74 specimens from 54 patients. Four Shigella spp. were recovered from four patients. Forty-seven of the 74 Salmonella spp. were recovered on both the primary xylose-lysine-deoxycholate (XLD) and after enrichment. No Salmonella or Shigella grew on the primary XLD only. Twenty-six Salmonella spp. were recovered only after selenite enrichment. Of these, 17 were from newly identified patients. The elimination of selenite enrichment would have significantly reduced our yield, whereas the elimination of the primary XLD would not have resulted in any fewer isolates and would have resulted in savings of approximately $4000 yearly.

摘要

有几份参考文献建议,亚硒酸盐增菌法仅用于来自可疑携带者的粪便培养、疫情期间以及其他特殊情况。为了确定这种方法的影响,我们检查了本实验室培养的3977份标本的结果。从医生和公共卫生部门收集了流行病学信息。在54名患者的74份标本中鉴定出沙门氏菌属。从4名患者中分离出4株志贺氏菌属。74株沙门氏菌属中的47株在初次木糖赖氨酸脱氧胆酸盐(XLD)培养基上以及增菌后均被检出。仅在初次XLD培养基上没有沙门氏菌或志贺氏菌生长。仅在亚硒酸盐增菌后分离出26株沙门氏菌属。其中,17株来自新确诊的患者。取消亚硒酸盐增菌会显著降低我们的检出率,而取消初次XLD培养基则不会减少分离株数量,并且每年可节省约4000美元。

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