Karolis C, Coster H G, Chilcott T C, Barrow K D
UNESCO Centre for Membrane Science and Technology and Department of Biophysics, School of Physics, University of New South Wales, Sydney, Australia.
Biochim Biophys Acta. 1998 Jan 19;1368(2):247-55. doi: 10.1016/s0005-2736(97)00180-6.
Low frequency impedance measurements of pure egg lecithin (phosphatidylcholine) bilayers have revealed the presence of four layers which can be attributed to the acyl chain, carbonyl, glycerol bridge and phosphatidylcholine regions of the lecithin molecule. Measurements on bilayers formed in the presence of unoxidised-cholesterol revealed that cholesterol molecules were located in the hydrocarbon region of the bilayer with its hydroxyl groups aligned with the carbonyl region of the lecithin molecules. Measurements of oxidised-cholesterol lecithin bilayers revealed that these molecules protruded less into the hydrocarbon region and their polar hydroxyl group aligned with the glycerol bridge region of the lecithin molecule.
对纯卵磷脂（磷脂酰胆碱）双层膜进行的低频阻抗测量显示存在四层结构，这四层可归因于卵磷脂分子的酰基链、羰基、甘油桥和磷脂酰胆碱区域。对在未氧化胆固醇存在下形成的双层膜进行的测量表明，胆固醇分子位于双层膜的烃区域，其羟基与卵磷脂分子的羰基区域对齐。对氧化胆固醇卵磷脂双层膜的测量表明，这些分子向烃区域的突出程度较小，并且它们的极性羟基与卵磷脂分子的甘油桥区域对齐。
