Pitha P M, Fernie B, Maldarelli F, Wivel N A
J Supramol Struct. 1979;12(1):35-46. doi: 10.1002/jss.400120105.
Interferon treatment of JLSV-6 cells chronically infected with Rauscher MuLV leads to the formation of noninfectious particles (interferon virions) containing the structural proteins of env and gag genes as well as additional viral polypeptides. In the control virions the major glycoprotein detected is gp71, interferon virions contain in addition to gp71 and 85k dalton (gp85) glucosamine-containing, fucose-deficient glycoprotein which is recognized by antiserum to MuLV but not by the gp71 antiserum. The surface iodination of the intact virions indicates that both gp71 and gp85 are the major components of the external virions envelope. However, unlike the control virions in which gp71 associates with p15E (gp90), the gp71-p15E complex was not detected in interferon virions. The analysis of the iodinated proteins of the disrupted interferon virions revealed the presence of 85k and 65k dalton polypeptides preciptable with antiserum against MuLV, which are not present in the control virions. The difference in the polypeptide pattern of virions produced in the presence of interferon does not seem to be a consequence of the slowdown in the synthesis of viral proteins or their processing in the interferon-treated cells. Both the structural proteins of env and gag genes seem to be synthesized and processed at a comparable rate in the interferon-treated and -untreated cells. These results indicate an alteration of virus assembly in the presence of interferon.
用干扰素处理长期感染劳斯氏鼠白血病病毒（Rauscher MuLV）的JLSV - 6细胞，会导致形成含有env和gag基因结构蛋白以及其他病毒多肽的非感染性颗粒（干扰素病毒粒子）。在对照病毒粒子中检测到的主要糖蛋白是gp71，干扰素病毒粒子除了含有gp71和85k道尔顿（gp85）的含葡糖胺、缺乏岩藻糖的糖蛋白外，该糖蛋白能被抗MuLV血清识别，但不能被gp71抗血清识别。完整病毒粒子的表面碘化表明，gp71和gp85都是病毒粒子外膜的主要成分。然而，与gp71与p15E（gp90）结合的对照病毒粒子不同，在干扰素病毒粒子中未检测到gp71 - p15E复合物。对破碎的干扰素病毒粒子的碘化蛋白分析显示，存在可被抗MuLV血清沉淀的85k和65k道尔顿的多肽，而对照病毒粒子中不存在这些多肽。在干扰素存在下产生的病毒粒子多肽模式的差异似乎不是病毒蛋白合成减慢或其在经干扰素处理的细胞中加工的结果。env和gag基因的结构蛋白在经干扰素处理和未处理的细胞中似乎以相当的速率合成和加工。这些结果表明在干扰素存在下病毒装配发生了改变。
