Tolnay E, Kuhnen C, Voss B, Wiethege T, Müller K M
Department of Pulmonology, Semmelweis Medical School, Budapest, Hungary.
Virchows Arch. 1998 Jan;432(1):61-5. doi: 10.1007/s004280050135.
Vascular endothelial growth factor (VEGF) is a multifunctional cytokine, which has recently been reported to enhance the activation and migration of monocytes through the flt receptor in vitro, which are key events in granuloma formation of granulomatous disorders and in sarcoidosis. Since activated macrophages and monocytes are known to be involved in sarcoid granuloma formation in sarcoidosis, we investigated the expression of VEGF and its receptor flt in 33 paraffin-embedded lung tissue biopsies of patients with pulmonary sarcoidosis. VEGF-mRNA was localized by nonradioactive in situ hybridization, VEGF and flt expression were visualized immunohistochemically. We found an increased transcription and protein production of VEGF and an overexpression of flt in activated alveolar macrophages, in epitheloid cells, and in multinuclear giant cells of pulmonary sarcoid granulomas.
血管内皮生长因子（VEGF）是一种多功能细胞因子，最近有报道称其在体外可通过flt受体增强单核细胞的活化和迁移，而这是肉芽肿性疾病和结节病肉芽肿形成过程中的关键事件。由于已知活化的巨噬细胞和单核细胞参与结节病中结节性肉芽肿的形成，我们研究了33例肺结节病患者石蜡包埋肺组织活检标本中VEGF及其受体flt的表达情况。通过非放射性原位杂交定位VEGF - mRNA，采用免疫组织化学方法观察VEGF和flt的表达。我们发现，在肺结节病肉芽肿的活化肺泡巨噬细胞、上皮样细胞和多核巨细胞中，VEGF的转录和蛋白生成增加，flt表达上调。
