Cox-Singh J, Mahayet S, Abdullah M S, Singh B
School of Medical Sciences, University of Science Malaysia, Kelantan, Malaysia.
Int J Parasitol. 1997 Dec;27(12):1575-7. doi: 10.1016/s0020-7519(97)00147-1.
Malaria remains a disease of underdeveloped and remote regions of the world. The application of polymerase chain reaction (PCR) technology to malaria epidemiology has the potential for increasing our knowledge and understanding of this disease. In order to study malaria in all geographical locations it is important that specimen collection and DNA extraction for PCR be kept simple. Here we report a method for extracting DNA from dried blood spots on filter paper which is capable of detecting one Plasmodium falciparum and two Plasmodium vivax parasites/microliter of whole blood by nested PCR without compromising the simplicity of specimen collection or DNA extraction.
疟疾仍然是世界上不发达和偏远地区的一种疾病。将聚合酶链反应（PCR）技术应用于疟疾流行病学，有可能增加我们对这种疾病的认识和理解。为了在所有地理位置研究疟疾，重要的是用于PCR的样本采集和DNA提取要保持简单。在这里，我们报告一种从滤纸上的干血斑中提取DNA的方法，该方法能够通过巢式PCR检测每微升全血中的一个恶性疟原虫和两个间日疟原虫寄生虫，同时不影响样本采集或DNA提取的简单性。
