Delahunty T J, Johnston J M
J Lipid Res. 1976 Mar;17(2):112-6.
The secretion of phosphatidylcholine was studied by incubating hamster lung slices which had been prelabeled by the in vivo administration of 14C-labeled choline. The release of 14C-labeled phospholipid into the medium continued for 2 hr. The specific activity of phosphatidylcholine in the medium was one-third that found in the tissue, and the fatty acid composition of the released phosphatidylcholine corresponded to that of surfactant. The prior injection of colchicine resulted in a 60% inhibition of phosphatidylcholine release into the incubation medium. A similar effect was obtained when vinblastine was administered. Colchicine demonstrated no inhibiting effect on the release of protein from lung slices which had been prelabeled by the administration of 14C-labeled leucine. The possible function of the microtubular system in the secretion of surface active components is discussed.
通过孵育经体内注射14C标记胆碱预标记的仓鼠肺切片，研究了磷脂酰胆碱的分泌情况。14C标记的磷脂向培养基中的释放持续了2小时。培养基中磷脂酰胆碱的比活性是组织中比活性的三分之一，释放的磷脂酰胆碱的脂肪酸组成与表面活性剂的脂肪酸组成相对应。预先注射秋水仙碱可使磷脂酰胆碱向孵育培养基中的释放受到60%的抑制。给予长春花碱时也获得了类似的效果。秋水仙碱对经注射14C标记亮氨酸预标记的肺切片中蛋白质的释放没有抑制作用。文中讨论了微管系统在表面活性成分分泌中的可能作用。
